438 
Biology of Txodidae 
Effect of the bite of I. putus. 
In Part I (pp. 84-85) the effects of the bite of a female were described. 
I was bitten on 13. vii. 1912, and recorded the after-effects up to 20. m. 
1913. The area about the seat of the bite remained discoloured until 
the spring of 1914, and it itched at intervals of about two weeks or 
more. The itching sensation has continiied to occur suddenly at 
intervals of a month or so but it now subsides rapidly. All signs of 
the bite have disappeared. The last dates on which the itching returned 
was on 9. xn. 1914, .3-5 and 20. i. 1915. 
RHIPICEPHALUS APPENDICULATUS. 
To the account of the biology of this species given in Part I 
{Parasitology, vi. pp. 111-118), the following note should be added 
regarding the 'proportion of the sexes observed among the progeny of an 
undetermined number of females of the strain received from South 
Africa in 1910. 
The ticks were fed as nymphs upon calves. 
No. of gorged 
riate when the 
No. of adults 
Proportions of 
Lot Xo. 
nymphs collected 
adults emerged 
of botli sexes 
each 
sex 
<? 
? 
1 
74 
26. I. 1913 
71 
34 
37 
2 
270 
4. II. 
82 
39 
43 
.3 
154 
24. III. 
151 
78 
73 
4 
218 
24. V. 
203 
105 
98 
5 
47 
29. VII. 
42 
16 
26 
549 
272 
277 
The numbers of each sex may therefore be regarded as equal. 
The loss of life during metamorphosis from nymph to adult is very 
slight in four out of the five observations; only in Lot 2 was there a 
considerable mortality amongst the nymphs. 
RHIPICEPHALUS BURSA. 
The ticks used for the study of the biology of this species were derived 
from Hammam Meskoutine, Algeria, whence I received (N. 1305 h) two 
gorged females that had been collected from sheep by M. Paul Dechabert 
at my request. The females laid numerous eggs whence larvae emerged 
on 5. IX. 1911, that is 50-51 days after the first eggs were laid. We have 
maintained the strain to date; all our stock is descended from one of 
the females before mentioned. 
