W. F. Cooper and H. E. Laws 
209 
Beast No. 2010. (J. 
Date 
1909 
Temperature 
Effect on ticks 
Remarks 
March 9th 
„ 15th 
o 
o 
0-5 gm. of As 2 0 3 , as sodium arse¬ 
nite, dissolved in 15 c.c. of water 
injected on escutcheon 
Skin badly swollen between hind¬ 
„ 19th 
101-8 „ 
Ticks within few inches 
legs, from anus to sheath 
Swelling incised and dressed with 
„ 23rd 
103-4 „ 
of needle-puncture all 
dead. Elsewhere ticks 
unaffected 
Ticks on body generally, 
carbolic acid and subsequently 
attended to daily 
Beast almost unable to walk 
„ 31st 
103-6 ,, 
unaffected 
All living ticks destroyed by 
.April 7 th 
application of oil 
Beast recovered. 
Appendix V. 
The Effect on Ticks of Arsenic in strong solution applied to 
a limited area of the skin. 
The following experiment was carried out with the object of deter¬ 
mining whether arsenic, applied in strong solution to a limited area 
of the skin, would he absorbed in sufficient quantity to destroy ticks 
distributed generally over the skin, or, in other words, whether such 
a solution is only effective in the area to which it is applied. 
A beast was taken and the skin of one side of the body was divided 
into two approximately equal halves by a vertical red paint line situated 
midway between the fore and hind legs. The fore quarter of the ox 
having been covered to prevent contamination, the hind quarter posterior 
to the paint line was carefully sprayed with a 2 per cent, solution of 
arsenious oxide as sodium arsenite. Such a solution is, of course, far 
too strong for an application to the entire skin surface and would cause 
the death of an animal so treated in a very short time 1 . Four days 
later, the skin of the sprayed area showed a marked glossiness and 
the ticks attached thereto -were dead. Later, symptoms of scalding 
developed, and in about three weeks the epidermis, with the hair attached, 
peeled off in sheets the size of a piece of note-paper. New hair ultimately 
appeared and recovery was apparently complete. The experiment was 
repeated twice, using less concentrated solutions, with identical results. 
1 The strongest solution ever advised is 0-25 per cent.; the strongest advocated by 
any Government is one containing 0-20 per cent, of As 2 0 3 . 
