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EXPERIMENTS IN THE “ TRYPOSAFROL ” TREAT¬ 
MENT OF TRYPANOSOMIASIS (T. BRUCEI) IN 
GUINEA-PIGS AND OF PIROPLASMOSIS IN 
DOGS. 
By G. H. F. NUTTALL, F.R.S. 
and E. HINDLE, Ph.D. 
(From the Quick Laboratory, University of Cambridge.) 
In 1912, Brieger and Krause reported upon experiments with a dye 
to which they gave the name of trypasafrol (since called tryposafrol). 
They stated that it cured Nagana in rats and guinea-pigs. The authors 
experimented with four laboratory strains of Trypanosoma brucei. The 
animals were treated immediately after inoculation or after the lapse 
of a day or two, the dye being given every other day in quantities of 
0-05 to 0-1 g. well mixed with the moistened food which consisted of 
bread and bran for rats and guinea-pigs respectively. On the alternate 
days the animals received “ Kraftfutter,” i.e. non-medicated food. 
On the occasion of the International Congress of Hygiene and 
Demography, held at Washington in September 1912, Geheimrat 
Brieger approached one of us (G. H. F. N.) with the suggestion that 
experiments with tryposafrol should be tried in Cambridge on some of 
the protozoal diseases upon the study of which we were engaged. 
Geheimrat Brieger, on his return to Berlin, sent a supply of the dye, 
and, in a letter dated 28. xi. 1912, wrote that the remedy had also given 
uniformly good results in the treatment of bovine piroplasmosis. In 
the treatment of cattle he recommended an initial dose of 0-25 g., 
followed on the next day by 0-5 g. and then 1-0 g. on each succeeding 
day, the whole amount being dissolved in 5 litres of water to which 
sugar or molasses was added. 
Initial experiments in the treatment of canine piroplasmosis and 
East Coast Fever of cattle were conducted in Cambridge but they 
yielded negative results. The experiments on East Coast Fever have 
recently been published by Nuttall (25. vi. 1915). 
