G-. H. F. Nuttall and E. Hindle 
219 
The glowing accounts given by Brieger and Krause of the cures they 
had effected with the dye in the treatment of experimental Nagana, 
soon led to its trial by other investigators. Rietz and Leupold (vn. 1913) 
reported adversely upon its efficacy in experimental Nagana and stated 
that tryposafrol exerts a toxic action. Brieger and Krause (1914) 
claim, however, that the experiments reported by Rietz did not fulfil 
the conditions required. Whereas the authors used guinea-pigs and 
rats, Rietz and Leupold experimented with mice coupled with highly 
virulent laboratory strains of T. brucei known as “ Ehrlich ferox” and 
“Morgenroth.” The authors deny that tryposafrol is toxic for they 
report having given 1 g. a day for 30 days to a man and for about 
50 days to a dog. Whereas tryposafrol may produce diarrhoea they 
find that novo-tryposafrol (an improved preparation) does not do so. 
The dye was next tried by others in the treatment of sleeping 
sickness in man. Lurz (ix. 1913) reported upon 22 cases treated by 
Wittrock and himself: Group I consisted of six patients in a relatively 
good condition; the patients received 0-25 g. tryposafrol per os as 
directed. When given on an empty stomach the dye produced nausea, 
and diarrhoea on the day following. The dye was best supported when 
given dry after meals. The urine and faeces were stained red from the 
second day onwards. The dye exerted no beneficial effect. Group II 
consisted of six patients. Acting on the advice of Brieger and Krause 
the dose was reduced to 0-1 g. daily for a week, after which the dosage 
was further decreased. Result negative. Group III consisted of ten 
patients who received 0-1 g. per day for 10 days, then 0-2 and 0-3 g. 
up to the 42nd day. Result negative. Lurz therefore concluded that 
tryposafrol, both in large and small doses, is useless in the treatment of 
sleeping sickness. 
Werner (iv. 1914) treated two cases of sleeping sickness in Europeans 
with tryposafrol supplied by Brieger and Krause. The dose ranged 
from 0-1 to 0-39 g. The dye caused diarrhoea and did not cure the 
patients. Similarly Mouchet and Dubois (1914), working in the Congo, 
state that the dye is useless in the treatment of human trypanosomiasis. 
Furthermore, in the paper by Brieger and Krause (1914), already 
quoted, the somewhat surprising statement is made that tryposafrol (or 
novo-tryposafrol) is capable of curing the most diverse diseases besides 
trypanosomiasis: a report reached the authors from Brazil of the 
successful treatment of a case of Leishmaniasis in man and good results 
have been obtained in the treatment of Foot and Mouth Disease in 
cattle, Swine Fever, and Distemper in dogs. Piroplasmosis in cattle, as 
it occurs in Europe, has been treated with brilliant results (“glanzende 
