222 
Tryposafrol 
coloured red by the dye from tbe third day 1 . Two guinea-pigs (Nos. 4 
and 5) were inoculated at the same time as the foregoing and received 
the same treatment except that it began on day 5 and, in the case of 
guinea-pig 5, the dose was increased to 0-1 g. beginning with day 28. 
(Novo-tryposafrol.) 
Weight Occurrence of trypanosomes in the blood on succeeding days, etc. 
220 g. 1 tryp. per field on day 5; 20-30 tryps. per field on day 6. Death on 
day 6. At autopsy the intestines were found inflamed and distended. 
215 g. Showed no tryps. in its blood but died on day 5. At autopsy the 
intestines were found ulcerated. 
220 g. 1 tryp. per blood-film on day 5; 0 on 6th; 2 per field on 7th; 6 and 
2 per film on 8tli and 9th; 0 on 10th and 11th; 4 per film on 12th 
and 13th; 1 per 4 fields on 14th; 1 per 2 fields on 15th; 15, 3, 4, 1, 
1 and 6 per field on 16th-21st; 1 per 2 fields on 22nd; 1,2 and 3 per 
film on 23rd-25th; 0 on 26th and 27th. Death on 27th day. 
195 g. 1 tryp. per blood-film on day 5; 1 per 2 fields on the 6th. Death on 
7th day. 
1 tryp. per 10 fields on day 5; 5, 40, 3 and 1 per field on 6th-9tb 
respectively; 6 per blood-film on 10th; 0 on 11th; 1 per film on 
12tli; 0 on 13th-16th; 1 per 2 fields on 17th; 1, 2 and 3 per field 
on 18th-20th; 1 per 3 fields on 21st; 2 per film on 22nd; 1 per 
4 fields on 23rd; 6, 1. 2, 6 and 1 per film on 24th-28th; 1, 2, 3 and 
10 per field on 29th-32nd. Death on the 33rd day. 
Six guinea-pigs (Nos. 6-11) were inoculated on 9. vi. 1914 (day 1) 
with heart blood of guinea-pig containing 25 T. brucei per field. Treat¬ 
ment began on the 3rd day, 0-1 g. of Tryposafrol being given daily in 
bran and water. The faeces appeared coloured from the 4th day. 
Guinea- 
( Tryposafrol .) 
pi£C 
No. 
Weight 
Occurrence of trypanosomes in the blood on succeeding days, etc. 
6 
220 g. 
4 tryps. per blood-film on days 4 and 5; 1, 45, 50 per field on 6th-8th ; 
numerous tryps. on 9th-11th. Death on 11th day. 
7 
260 g. 
6 tryps. per film on day 4; 1 and 6 per field on 5th and 6th respectively. 
Death on 7th day. 
8 
225 g. 
4 tryps. per film on day 4; 1 and 6 per field on 5th and 6th. Death on 
7th day. 
9 
250 g. 
1 tryp. per film on day 4; 1 and 12 per field on 5th and 6th. Death 
on 7th day. 
10 
230 g. 
2 tryps. per film on day 4; 8 per film on 5th; 5 per field on 6th; 
numerous tryps. on 7th. Death on 7th day. 
11 
215 g. 
3 tryps. per film on days 4 and 5; 3 per field on 6th; numerous tryps. 
on 7th and 8th. Death on 8th day. 
1 At autopsy. 
in all our experimental animals, the dye was found confined to the 
intestine: 
; it does not cause diffuse staining like trypanblue. 
Guinea- 
pig 
No. 
1 
2 
3 
