G. H. F. Nutt all and E. Hindle 
223 
Control Experiments. 
Three guinea-pigs (A, B and C) served as controls to the treated 
guinea-pigs Nos. 1-11. They were inoculated on the same day as 
guinea-pigs 1-5 with the same amount of infected blood; the animals 
received no treatment. 
Guinea- 
pig Weight Occurrence of trypanosomes in the blood on succeeding days, etc. 
A 190 g. 1 tryp. per field on day 5; 10 on 6th ; many on 10th-l 1th ; 15-20 on 
12th-14th; 1 per 2 fields on 15th; 8, 30, 25, 50 on 16th-19th; many 
on 21st-23rd. Death on 23rd day. 
B 235 g. 2 tryps. per field on day 5; 30 on 6th; many on 10th; 1 on 11th; 
2 and 6 per film on 9th and 10th; on the llth-23rd the number of 
tryps. per field varied as follows: 6, 8, 3, 30, 10, 1, 10, 4, 1, 2, 4, 40, 
6; 1 tryp. per 4 fields on 24th; on the 25th-35tb their number per 
field numbered each day 1, 2, 2, 1, 1, 3, 3, 6, 6, 4 and 30 respectively; 
numerous on the 36th; on the 37th-40th they numbered 10, 1, 6, 50 
per field; numerous on 41st-44th. Death on 44th day. 
C 210 g. 1 tryp. per field on day 5; 12 on 6th; numerous on 7th-8th; on the 
9th-16th they numbered 20, 1, 2, 45, 1. 1, 12 and 50 per field respec¬ 
tively; 6 per film on 17th; on the 18th-23rd there were 6, 10, l, 
4, 20 and 6 per field respectively; 4 per film on 24th; on the 25th-27th 
there were 1, 4 and 6 per field respectively; numerous on 28th-29th; 
on the 30th-32nd there were 20, 40 and 40 per field respectively; 
numerous on 23rd; 1 and 4 per film on 34th—35th; on the 36th-39th 
there were 1, 20, 40 and 30 per field respectively; numerous on 
40th-45th. Death on 45th day. 
2nd Series. 
Four guinea-pigs (Nos. 12-15) were inoculated subcutaneously on 
10. vii. 1914 (day 1) with guinea-pig blood containing 10 T. brucei per 
field. Treatment began on tbe day of inoculation; 0-1 g. of novo- 
tryposafrol was given daily in bran and water. Tbe faeces were coloured 
with the dye from the 4th day. 
Four guinea-pigs (Nos. J.6-19) inoculated at the same time and in 
the same manner as Nos. 12-15. Treatment began on the day of 
inoculation; 0-1 g. of tryposafrol was given daily in bran and water. 
The faeces became coloured as in the others. 
