G. H. F. Nutt all and E. Hindlb 
227 
Dog A (control). Collie, weight 38 lbs. Not treated. 
Temp. ‘P. 
Day a.m. p.m. 
1 
The dog was inoculated 
2 
3 
4 
5 
6 
101-5 
102-6 
Parasites appear in dog’s blood 
7 
105 
104-2 
Parasites increasing. Fever began 
8 
104-2 
104-6 
Weight 37 lbs. 
9 
105-4 
104-8 
10 
105 
104-4 
Dog not feeding 
11 
105-4 
105-1 
JJ JJ 
12 
105 
105-2 
„ ,, anaemic, haemoglobinuria 
13 
105-2 
„ „ Dog died of piroplasmosis 
Summary and Conclusions. 
All of our guinea-pigs infected with Trypanosoma brucei (strain 
“ ferox ”) died whether they were treated or not. It is evident that both 
tryposafrol and novo-tryposafrol exerted a directly injurious effect upon 
the guinea-pigs. Reckoning the day on which the guinea-pigs were 
inoculated as day 1, the 19 treated guinea-pigs died respectively on 
days 5, 6, 7, 7, 7, 7, 7, 7, 8, 8, 8, 8, 11, 18, 19, 19, 26, 27 and 33. The 
six untreated guinea-pigs died respectively on days 23, 25, 25, 27, 44 and 
45. The two preparations of the dye are therefore worse than useless 
as remedies for Nagana in guinea-pigs. 
Five dogs were infected with Piroplasma canis (Cambridge strain) 
of which four were treated and one not treated with novo-tryposafrol. 
All of the dogs died although treatment was given under the most favour¬ 
able conditions, starting on the day of inoculation. The four treated 
dogs died on days 12, 9, 17 and 12 after inoculation respectively; the 
untreated (control) dog died on the 13th day. The drug exerted no 
influence upon the course of the disease, nor upon the appearance of the 
parasites and their progressive increase in the blood. Novo-tryposafrol 
may therefore be regarded as useless in the treatment of canine piro- 
plasmosis, and, judged from these results on dogs, it will no doubt prove 
