10 
of recombinant DNA facilities and practices. However, in order to permit 
greater flexibility in scaled-up procedures, he suggested that Section III-B-51 
be modified to read: 
" III-B-5. Experiments Involving ftore than 10 Liters of Culture . 
The appropriate containment will be decided by the IBC except vhere 
exempted under Section III-D-5. Where appropriate, the large-scale 
containment recommendations of the NIH should be used (45 FR 24968)." 
The exception specified in Appendix C (Section C-II, C-III, and C-IV) which 
deals with large-scale procedures using EL coli K-12 , Saccharomyces cerevisiae 
and Bacillus subtilis host-vector systems, would be modified to require IBC 
notification simultaneously with the initiation of large-scale experiments 
when IBC-approved Pl-LS containment facilities are used . Sections C-II, 
C-III, and C-IV would be modified to read: 
"Large-scale experiments (i.e., more than 10 liters of culture) 
require IBC notice simultaneously with the initiation of exper- 
iments where IBC-approved practices and an IBC-approved Pl-LS 
containment facility will be used. Where these conditions are 
not satisfied, refer to Part III-B-5." 
Proposals Concerning the Physical Containment Recommendations for Large-Scale 
Uses of Organisms Containing Recombinant EWA Molecules 
Er. Waitz then proposed modification of Sections VII-B-1, VII-C-1, and VII-D-l 
of the Physical Containment Recommendations for Large-Scale Uses of Organisms 
Containing Recombinant DLPv Molecules. Ihe language of these sections reads as 
follows: 
T47] 
