Federal Register / Vol. 47, No. 184 / Wednesday, September 22, 1982 / Notices 
41925 
structural gene of the s/)/£a-like toxin of 
Escherichia coli. The E. coli shiga-like 
toxin has biological activity similar to 
the activity of Shigella dysenteriae 
neurotoxin. The shiga-like toxin gene 
would be cloned in E. coli EKl host- 
vector systems using plasmid, cosmid, or 
lambda cloning vectors. 
III. Request To Release Strains of 
Pseudomonas Syringae and Erwinia 
Herbicola 
Drs. Nicholas Panopoulos and Steven 
Lindow of the University of California, 
Berkeley, request permission to 
construct and release Pseudomonas 
syringae pv. syringae and Erwinia 
herbicola carrying in vitro generated 
deletions of all or part of the genes 
involved in ice nucleation for purposes 
of biological control of frost damage in 
plants. 
IV. Request To Field-Test Transformed 
Tomato and Tobacco Plants 
Dr. John Sanford of Cornell University 
requests permission to field-test tomato 
and tobacco plants that will be 
transformed with bacterial and yeast 
DNA using pollen as a vector. Plants 
will be screened in the field to detect 
transformation events. 
V. Proposed Revision of Appendix F 
NIH staff proposes to revise Appendix 
F, Section F-l, second sentence, to 
clarify that the subject experiments are 
not in fact “prohibited," but rather full 
under Section III— A of the Guidelines, 
which requires that the experiments 
receive RAC review and Nil! and IBC 
approval before initiation. The current 
language is inaccurate. 
The relevant sentence in Appendix F 
currently reads as follows: “Cloning of 
genes coding for molecules toxic for 
vertebrates that have an LD M of less 
than 100 nanograms per kilogram body 
weight [e.g., microbial toxins such as the 
botulinum toxins, tetanus toxin, 
diphtheria toxin, Shigella dysenteriae 
neurotoxin] is prohibited." 
NIH staff proposes that this sentence 
be amended to be read as follows: “The 
cloning of genes coding for molecules 
toxic for vertebrates that have and 
LD 50 of less than 100 nanograms per 
kilogram body weight [e.g., microbial 
toxins such as the botulinum toxins, 
tetanus toxin, diphtheria toxin, Shigella 
dysenteriae neurotoxin] is covered 
under Section III— A— 1 of the Guidelines 
and requires RAC review and NIH and 
IBC approval before initiation." 
Dated: September 14. 1982. 
Bernard Talbot, 
Acting Director, National Institute of Allergy 
and Infectious Diseases, National Institutes of 
Health. 
OMB’s “Mandatory Information 
Requirements for Federal Assistance 
Program Announcements" (45 FR 39592) 
requires a statement concerning the 
official government programs contained 
in the Catalog of Federal Domestic 
Assistance. Normally NIH lists in its 
announcements the number and title of 
affected individual programs for the 
guidance of the public. Because the 
guidance in this notice covers not only 
virtually every NIH program but also 
essentially every federal research 
program in which DNA recombinant 
molecule techniques could be used, it 
has been determined to be not cost 
effective or in the public interest to 
attempt to list these programs. Such a 
list would likely require several 
additional pages. In addition, NIH could 
not be certain that every federal 
program would be included as many 
federal agencies, as well as private 
organizations, both national and 
international, have elected to follow the 
NIH Guidelines. In lieu of the individual 
program listing, NIH invites readers to 
direct questions to the information 
address above about whether individual 
programs listed in the Catalog of 
Federal Domestic Assistance are 
affected. 
NIH programs are not coverd by OMB 
Circular A-95 because they fit the 
description of "programs not considered 
appropriate" in Section 8— (b) — (4) and (5) 
of that Circular. 
|FR Doc. 82-28010 Piled 9-21-82; 8:4$ &m| 
BILUNG COO€ 4149-01-84 
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