Federal Register / Vol. 48, No. 106 / Wednesday, June 1, 1983 / Notices 
24565 
Sublist C 
1. Streptomyces aureofaciens 
2. Streptomyces rimosus. 
3. Streptomyces coelicolor 
Sublist O 
1. Streptomyces griseus. 
2. Streptomyces cyaneus. 
3. Streptomyces vcnezue/ae. 
Sublist E 
One way transfer of Streptococcus niutans 
■or Streptococcus lactis DNA into 
Streptococcus sanguis. 
Sublist F 
1. Streptococcus sanguis. 
2. Streptococcus pneumqnioe. 
3. Streptococcus faecalis. 
4. Streptococcus pyogenes. 
Appendix B — Classification of 
Microorganisms of the Basis of Hazard 
Appendix B — I. Classification of 
Etiologic Agents. (The original reference 
for this classification was the 
publication "Classification of Etiological 
Agents on the Basis of Hazard," 4th 
edition, July 1974, U.S. Department of 
Health, Education, and Welfare, Public 
Health Service, Center for Disease 
Control, Office of Biosafety, Atlanta, 
Georgia 30333. For the purposes of these 
Guidelines, this list has been revised by 
the NIH.) 
Appendix B-I-A. Class 1 Agents. All 
bacterial, parasitic, fungal, viral, 
rickettsial, and chlamydial agents not 
included in higher classes. 
Appendix B-I-B. Class 2 Agents. 
Appendix B-I-B-l. Bacteria! Agents. 
Acinetobocter calcoaceticus 
Aclinobaci/lus — all species 
Aeromonas hydrophi/a 
Arizona hinshawii — all serotypes 
Bacillus anthracis 
Bordetella — all species 
Borrelia recurrentis, B. vincenli 
Campylobacter fetus 
Campylobacter jejuni 
Chlamydia psittaci 
Chlamydia trachomatis 
Clostridium botulinum : 
Cl. chauvoei, Cl. haemolyticum, 
Cl. histolyticum. Cl. novyi, 
Cl. septicum. Cl. tetani 
Corynebacterium diphtheriae: 
C. equi, C. haemolyticum , 
C. pseudotuberculosis, 
C. pyogenes, C. rena/e 
Edwardsiclla tarda 
Erysipelothrix insidiosa 
Escherichia coli — all enteropathogcnic, 
enterotoxigenic, enteroinvasive and strains 
bearing Kl antigen 
Haemophilus ducreyi, H. influenzae 
Klebsiella — all species and all serotypes 
Legionella pneumophila 
Leptospira interrogans — all serotypes 
Listeria — all species 
Moraxella—a\\ species 
Mycobacteria — all species except those 
listed in Class 3 
Mycoplasma — all species except 
Mycoplasma mycoides and Mycoplasma 
agalactiae, which are in Class 5 
Neisseria gonorrhoeae, N. meningitidis 
Pasteurella — all species except those listed in 
Class 3 
Salmonella — all species and all serotypes 
Shigella — all species and all serotypes 
Sphaerophorus necrophorus 
Staphylococcus aureus 
Streptobacillus moniliformis 
Streptococcus pneumoniae 
Streptococcus pyogenes 
Treponema carateum, T. pallidum, and T. 
pertenue 
Vibrio cholerae 
Vibrio parahemolyticus 
Yersinia enterocolitica 
Appendix B-I-B-2. Fungal Agents. 
Actinomycetes (including Nocardia species 
and Actinomyces species and Arachnia 
prop ion ica ) [2| 
Blastomyces dermatitidis 
Cryptococcus neoformans 
Pa racoccidioides braziliensis 
Appendix B-I-B-3. Parasitic Agents. 
Endamoeba histolytica 
Leishmania sp. 
Naegleria gruberi 
Schistosoma mansoni 
Toxoplasma gondii 
Toxocara can is 
Trichine/la spiralis 
Trypanosoma cruzi 
Appendix B-I-B-4. Viral, Rickettsial, 
and Chlamydial Agents. 
Adenoviruses — human — all types 
Cache Valley virus 
Coxsackie A and B viruses 
Cytomegalo viruses 
Echoviruses — all types 
Encephalomyocarditis virus [EMC] 
Flanders virus 
Hart Park virus 
Hepatitis — associated antigen material 
Herpes viruses — except Herpesvirus simiae 
( Monkey B virus ) which is in Class 4 
Corona viruses 
Influenza viruses — all types except A/PR8/ 
34, which is in Class 1 
Langat virus 
Lymphogranuloma venereum agent 
Measles virus 
Mumps virus 
Parainfluenza virus — all types except 
Parainfluenza virus 3, SF4 strain, which is 
in Class 1 
Polioviruses — all types, wild and attenuated 
Poxviruses — nil types except Alastrim, 
Smallpox, and Whitepox, which are Class 
5 and Monkey pox. which depending on 
experiments, is in Class 3 or Class 4 
Babies virus — all strains except Rabies street 
virus, which should be classified in Class 3 
Reoviruses — all types 
Respiratory syncytial virus 
Rhinoviruses — all types 
Rubella virus 
Simian viruses — all types except Herpesvirus 
simiae (Monkey B virus) and Marburg 
virus, which are in Class 4 
Sindbis virus 
Tensow virus 
Turlock virus 
Vaccinia virus 
Varicella virus 
Vesicular stomatitis virus [3| 
Vole rickettsia 
Yellow fever virus, 17D vaccine strain 
Appendix B-I-C. Class 3 Agents. 
Appendix B-I-C-l. Bacteria! Agents. 
Bartonella — all species 
Brucella — all species 
Francisella tularensis 
Mycobacterium avium. M. bovis, M. 
tuberculosis 
Pasteurella mullocide type B ("buffalo" and 
other foreign virulent strains) [3) 
Pseudomonas mallei |3| 
Pseudomonas pseudomallei (3) 
Yersinia pestis 
Appendix B-I-C-2. Fungal Agents. 
Coccidioides immitis 
Histoplosma capsulatum 
Histoplasma capsulatum var. duhoisii 
Appendix B-I-C-3. Parasitic Agents. 
None 
Appendix B-l-C— 4. Viral, Rickettsial, 
and Chlamydial Agents. 
Monkey pox, when used in vitro [4 ] 
Arboviruses — all strains except those in 
Class 2 and 4 [Arboviruses indigenous to 
the United States are in Class 3, except 
those listed in Class 2. West Nile and 
Semliki Forest viruses may be classified up 
or down, depending on the conditions of 
use and geographical locution of the 
laboratory.) 
Dengue virus, when used for transmission or 
animal inoculation experiments 
Lymphocytic choriomeningitis viris (LCM) 
Rabies street virus 
Rickettsia — all species except Vole rickettsia 
when used for transmission or animal 
inoculation experiments 
Yellow fever virus — wild, when used in vitro 
Appendix B-I-D. Class 4 Agents. 
Appendix B-I-D-l. Bacteria! Agents. 
None. 
Appendix B-I-D-2. Fungal Agents. 
None. 
Appendix B-I-D-3. Parasitic Agents. 
None. 
Appendix B-I-D— 4. Viral, Rickettsial, 
and Chlamydial Agents. 
Ebola fever virus 
Monkey pox, when used for transmission or 
animal inoculation experiments (4) 
Hemorrhagic fever agents, including Crimean 
hemorrhagic fever, (Congo). Junin. and 
Machupo viruses, and others as yet 
undefined 
Herpesvirus simiae (Monkey B virus) 
Lasso virus 
Marburg virus 
Tick-borne encephalitis virus complex. 
including Russian spring-summer 
encephalitis. Kyasanur forest disease, 
Omsk hemorrhagic fever, and Central 
European encephalitis viruses 
ri45i 
