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Federal Register / Vol. 48, No. 100 / Wednesday, June 1, 1983 / Notices 
an open front with inward air flow 1 for 
personnel protection, and HF.PA filtered mass 
recirculation air flow for product protection. 
The cabinet exhaust air is filtered through • 
IIEI’A filter. The face velocity of the inward 
flow of air through the full-width open front is 
75 feet per minute or greater. Design and 
peformance specifications for Class // 
cabinets have been adopted by the National 
Sanitation Foundation. Ann Arbor, Michigan. 
A Class III cabinet is a closed-front 
ventilated cabinet of gas-tight construction 
which provides the highest level of personnel 
protection of all biohazard safety cabinets. 
The interior of the cabinet is protected from 
contaminants exterior to the cabinet. The 
cabinet is fitted with arm-length rubber 
gloves and is operated under a negative 
pressure of at least 0.5 inches water gauge. 
All supply air is filtered through HFPA filters. 
Exhaust air is filtered through two HEPA 
filters or one HEPA filter and incinerator 
before being discharged to the outside 
environment. 
Appendix H — Shipment 
Recombinant DNA molecules 
contained in an organism or virus shall 
be shipped only as an etiologic agent 
under requirements of the U.S. Public 
Health Service, and the U.S. Department 
of Transportation (Section 72.3, Part 72, 
Title 42, and Sections 173.386-.388, Part 
173, Title 49, United States Code of 
Federal Regulations (CFR)) as specified 
below: 
Appendix H-I. Recombinant DNA 
molecules contained in an organism or 
virus requiring Pi, P2, or P3 physical 
containment, when offered for 
transportation or transported, are 
subject to all requirements of Section 
72.3(a )— (3). Part 72. Title 42 CFR. and 
Sections 173.386-.388, Part 173, Title 49 
CFR. 
Appendix H— II. Recombinant DNA 
molecules contained in an organism or 
virus requiring P4 physical containment, 
when offered for transportation or 
transported, are subject to the 
requirements listed above under 
Appendix H-l and are also subject to 
Section 72.3(f), Part 72, Title 42 CFR. 
Appendix H— III. Information on 
packaging and labeling of etiologic 
agents is shown in Figures 1, 2, and 3. 
Additional information on packaging 
and shipment is given in the 
“Laboratory Safety Monograph — A 
Supplement to the NIH Guidelines for 
Recombinant DNA Research," available 
from ORDA. 
BILLING COOE 4140-01-M 
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