13 
Dr. Joklik who had seconded Dr. Levine's first motion accepted this modified 
motion. 
Dr. Martin asked if Dr. Levine had intentionally restricted this motion to 
research performed by Drs. O'Brien and co-workers. Dr. Levine replied that 
he had as Dr. O'Brien's group is the premier group in the field. 
By a vote of nine in favor, five opposed, and four abstentions, the RAC 
recommended acceptance of Dr. Levine's motion. 
Dr. Wens ink moved the same motion with the names of the researchers deleted 
from the motion. By a vote of eight in favor, four opposed, and five 
abstentions, the RAC recommended acceptance of Dr. Wensink's motion. 
IV. PI CCWIAILMENT CONDITIONS FOR PLANTS 
Dr. Tolin said several inquiries concerning the specifications for contained 
growth conditions for plants have been received from Institutional Biosafety 
Committees (IBCs) . In order to facilitate understanding of these require- 
ments, Dr. Tblin, the US DA representative to the RAC, suggested that 
language (tabs 1133/VI, 1138) describing the specifications for contained 
growth conditions for plants be added to the Guidelines. One mechanism by 
which to include such a description in the Guidelines would be for Section 
III-B-4-b to be footnoted. 
Section III-B-4-b reads as follows: 
"III-B-4-b. For all experiments involving whole animals and plants and 
not covered by III-B-4-a, the appropriate containment will be deter- 
mined by the IBC." 
A footnote concerning PI growth conditions for plants would be added to 
Section V, Footnotes and Reference of Section I-IV , as follows: 
"If the IBC wishes to require PI growth conditions for plants, 
this can be met by either: (1) a limited access greenhouse, or 
(2) a plant growth chamber, which are insect -restrictive and 
in which a pest control regime is maintained. Hie greenhouse 
or growth chamber should be under positive air pressure unless 
airborne propagules are generated (e.g. , fungal spores) in which 
case negative air pressure should be employed. Sterilization 
of run-off water is required only where this is a plausible 
route for dissemination of viable microorganisms containing 
recombinant DNA. Soil, plant parts, and unwanted plant materials 
shall be sterilized before disposal. Plant materials which 
have to be removed from the greenhouse or cabinet for further 
research shall be maintained under good laboratory practices 
as applied to plants. Plants can be transported locally, e.g., 
[258] 
