11 
"The Pl-LS level is recommended for experiments involving exempt host- 
vector systems described in Appendix C. " 
Or. Milewski suggested that the language might read: 
"For experiments involving exenpt host-vector systems, see Appendix C. " 
She suggested a second possibility would be to add the phrase "see Appendix C" 
after the third sentence of Appendix K-I. Drs. McKinney and Tolin agreed. 
Dr. Tolin then read her three part motion: 
1. The second paragraph of Appendix C-II, Experiments Involving E. coli 
K-12 Host-Vector Systems; Appendix C-II I, Experiments Involving Saccharo- 
myces cerevisiae Host -Vector Systems ; and Appendix C-IV, E xperiments 
Involving Bacillus suhtilis Host-Vector Systems ; would read as follows: 
"For these exempt laboratory experiments, PI physical containment condi- 
tions are recommended." 
2. A paragraph would be added following the second paragraph of Appendix C-II, 
Appendix C-II-I, and Appendix C-IV. That paragraph would read as follows: 
"For large-scale fermentation experiments Pl-LS physical containment 
conditions are recommended. . However, following review by the IBC of 
appropriate data for a particular host-vector system, same latitude in 
the application of Pl-LS requirements as outlined in Appendix K-I I -A 
through K-II-F is permitted provided operator exposure and environmental 
losses are minimized." 
3. Additional language would be added to the fourth sentence of Appendix K-I, 
Selection of Physical Containment Levels . That sentence would read as 
follows : 
"The Pl-IS level of physical containment is required for large-scale 
research or production of viable organisms containing recombinant DMA 
molecules which require PI containment at the laboratory scale (See 
Appendix C) . " 
Mr. Barbeito suggested the word "personnel" be substituted for "operator." 
Personnel would encompass all workers in the facility as well as people walking 
in the street. Dr. Muth said any individual working in the Eli Lilly production 
area has operator training; these individuals might include maintenance, house- 
keeping personnel, pecple who transport materials, etc. Dr. Tolin accepted 
Mr. Barbeito' s proposed modification. 
Dr. McKinney suggested that the phrase "personnel and environmental exposures 
be rminimized" be substituted for the phrase "operator exposure and environ- 
mental losses are minimized. Dr. Levin questioned the definition of "minimized." 
[347] 
