12 
Dr. McGarrity asked what constituted "environmental loss." Dr. Muth said 
environmental loss referred primarily to aerosols; it could occur at exhaust 
vents or when materials are transferred frcm vessel to vessel. Dr. Tolin said 
she would delete the phrase "provided personnel and envir omental exposures 
are minimized" from her proposed motion. 
Dr. Mahaffey expressed concern about the protection afforded operators should 
the word "personnel" be deleted . 
Dr. Miller seconded Dr. Tolin' s motion. Dr. McGarrity called the question. 
Dr. McKinney reminded the group that any language accepted by the working 
group would be a recommend ation to the RAC. The language would be published 
in the Federal Register , considered by RAC and acted on by the NIH. By a vote 
of six in favor, none opposed and no abstentions, the working group accepted 
Dr. Tolin' s motion vhich reads as follows: 
"1. The second paragraph of Appendix C-II, Experiments Involving E. coli 
K-12 Host Vector Systems ; Appendix C-III, Experiments Involving 
Saccharcrryces cerevisiae Host-Vector Systems ; and Appendix C-IV, 
Experiments Involving Bacillus subtilis Host-Vector Systems ; would be 
modified to read as follows : 
'For these exempt laboratory experiments, PI physical containment 
conditions are recommended . ' 
"2. A paragraph would be added following the second paragraph of Appendix 
C-II, Appendix C-III, and Appendix C-IV. That paragraph would read 
as follows: 
'For large-scale fermentation experiments Pl-LS physical contain- 
ment conditions are recommended . However, following review by 
the IBC of appropriate data for a particular host-vector system, 
seme latitude in the application of Pl-LS requirements as outline 
in Appendix K-II-A through K-II-F is permitted.' 
"3. Additional language would be added to the fourth sentence of Appendix 
K-I, Selection of Physical Containment Levels . That sentence would read 
as follows: 
'The Pl-LS level of physical containment is required for large- 
scale research or production of viable organisms containing 
recombinant ENA molecules vhich require PI containment at the 
laboratory scale (See Appendix C) . ' " 
Dr. Miller then moved that the word "minimizes" be substituted for the word 
"prevents" in Appendix K-II-D of Appendix K-II, Pl-LS Level . Dr. Miller inter- 
preted minimize to mean the greatest efficiency obtainable by a recognized 
procedure. Dr. McGarrity seconded the motion. 
[348] 
