16 
Hr. Amtzen said DNA transfer will occur with many of the vectors used to 
transform plants. He offered the example of Cauliflower Mosaic Virus (CMV) 
engineered to express antibiotic resistance. When the plant is infected with 
the CMV vector, resistance to the antibiotic becomes systemic. The vector 
probably is present when the plant dies and decays. Dr. Arntzen did not feel 
this process would present hazard and felt the public should be educated as to 
the safety of these procedures. 
The working group agreed on the following language: 
" Items for Consideration to be Included in Proposal Submissions Under Appendix L . 
"These annotated items were presented for consideration by prospective 
proposal submitters to facilitate the process of approval. The working 
group has found that the proposals so far submitted for their consideration 
have emitted information that is considered minimal and essential for their 
approval. Basically, the group would like to see detailed objectives, 
materials, and methods, including methodology for monitoring the experiments, 
and expected results. At a minimum, summary data should be submitted to 
support the proposal. A check list of detailed requirements should include 
but is not limited to: 
"A. Description of Plant Materials. 
"1. Give cannon and scientific names of plants and cultivars, if 
appropriate. 'Tomato plants will be inoculated' is insufficient. 
"2. If appropriate, give data or information on the relative homogeneity 
of the plant cultivar, and specific genetic markers the cultivar is 
known to possess. 
"R. Vectors and Method of Introduction. 
"1. Describe the cloned DMA segment and its expression in the new host. 
"2. Give the method(s) by vhich proposed DNA vector will be or has been 
constructed . Diagrams are very helpful and may be necessary for 
adequate understanding of the construct. Explain the advantages 
(and disadvantage ( s) , if appropriate) of your vectors, if other 
candidate vectors could be considered. 
"3. If microorganisms are used to introduce vectors or are vectors 
themselves, indicate how they compare with wild-type strains. 
If disabled pathogens are used to transmit the vector, indicate 
measures that will most likely prevent these microorganisms from 
regaining or acquiring pathogenic potential. If the vector is 
likely to survive independently of the hosts, refer to this 
possibility and provide any available data to assess the probabi- 
lity of such transfer to likely organisms. 
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