Attachment II - Page 1 
PWG Guidelines for Proposal Submission 
These annotated guidelines are presented for consideration by 
prospective proposal submitters to facilitate the process of approval. 
The PWG has found that the proposals so far submitted for their consider- 
ation have omitted information that is considered minimal and essential 
for their approval. Basically, the group would like to see detailed 
objectives, materials and methods, including methodology for monitoring 
the experiments, and expected results. At least summary data should be 
submitted to support the proposal. A check list of detailed requirements 
should include, but is not limited to: 
1. Give common and scientific names of plants and cultivars, if 
appropriate. 
'Tomato plants will be inoculated' is insufficient. 
2. If appropriate, give data or information on the relative 
homogeneity of the plant cultivar, and specific genetic markers 
the cultivar is known to possess . 
3. Give specific strain designations of those you expect to use. 
'Some strains of Agrobaatevium rhizogenes will be used...' is 
insufficient. 
4. Give the method(s) by which the vector will be or is constructed. 
Diagrams are very helpful and may be necessary for adequate under- 
standing of the construct. Explain the advantages (and disadvantage (s) , 
if appropriate) of your vectors, if other candidate vectors could be 
considered. 
5. If live host microorganisms are used to introduce vectors or are 
vectors themselves. Indicate how they compare with wild-type strains. 
If disabled pathogens or vectors are used as hosts, indicate measures 
that will most likely prevent these microorganisms from regaining 
or acquiring pathogenic potential. 
6. Give criteria and methods by which the host microorganism will be 
monitored. If live host microorganisms are required to be present 
in field trials, indicate the means of strain identification and 
retrieval. If microorganisms are used to Introduce vectors, the 
assessment of subsequent absence of the microorganisms should be 
specified. 
7. If the microorganisms are transfer deficient, provide some documenta- 
tion either via the proposal or appropriate references. 
8. If the vectors are transfer deficient, provide some documentation 
via the proposal or appropriate references. 
9. If the vector is likely to survive independently of the hosts, 
refer to this possibility; if the answer is in the realm of reasonably 
high probability, provide data to assess such transfer to likely 
microorganisms. 
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