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Attachment II - Page 2 
10. Provide data from greenhouse and/or growth chamber studies under 
simulated field conditions to support prospective field studies. 
11. If appropriate, provide data on engineered plants and controls fed 
to test animals, such as mice. 
12. Provide data for field plot design on the following: 
a. total area 
b. location: where, how many 
c. plot design: replication, row spacing, planting, border rows, etc. 
d. name cul tiva r(s) , if appropriate 
e. specify plant monitoring procedures: frequency; types of data to 
be obtained , including leaf, seed, fruit, or root characteristics ; 
abnormal i ties , such as diseases; insect population monitoring; 
collection of meteorological data, etc.; types of data to be sought, 
such as yield, resistance to stress , lodging, etc. 
f. specify monitoring of the vector and/or introduced DNA 
g. specify access and security measures 
13. List qualifications of people involved in the experiments. 
NOTE: Appendix L (Release into the environment of certain plants. Federal 
Register 48: 24549 and 48: 37199) should be consulted for general 
guidelines regarding plants. 
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