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Federal Register / Vol. -19, No. 80 / Tuesday. April 24, 1904 / Notices 
discretion of the laboratory director when 
experiments ere in progress. 
Appendix G-H-A -1-b. Work *>ur!.,<:es ere 
decontaminated or.ce a day end after any 
spill of viable material. 
Appendix C-ll-A-I-c. All contaminated 
liquid nr sulid'wastes are decontaminated 
before disposal. 
Appendix G-U-A-t-d. Mechanical 
pipetting devices are used; mouth pipetting is 
prohibited. 
Appendix G-H-A-L-e. Eating, drinking, 
smoking, and applying cosmetics are not 
permitted in the work area Food may be 
stored in cabinets or refrigerators designated 
and used for this purpose only. 
Appendix G-ll-A-l-f. Persons wash their 
hands after they handle materials involving 
organisms containing recombinant DN’A 
molecules, and animals, and before leaving 
the laboratory. 
Appendix G-ll-A-I-g. All procedures are 
performed carefully to minimize the creation 
of aerosols. 
Appendix G-ll-A-l-b. Il is recommended 
that laboratory coats, gowns, or uniforms be 
worn to prevent contamination or soiling of 
9treet clothes. 
Appendix G-U-A-2. Special Practices. 
Appendix G-ll-A-2-a. Contaminated 
materials that are to be decontaminated at a 
site away from the laboratory are placed in a 
durable leakproof container which is closed 
before being removed from the laboratory. 
Appendix G-ll-A-2-b. An insect and 
rodent control program is in effect. 
Appendix G—H-A-3. Containment 
Equipment. 
Appendix G-H-A-3-a. Special containment 
equipment is generally not required for 
manipulations of agents assigned to Biosafety 
I-evel 1. 
Appendix G-II-A-4. Laboratory' Facilities. 
Appendix G-H-A—4—a. The laboratory Is 
designed so that it can be easily cleaned. 
Appendix G-H-A—4-b. Bench tops are 
impervious to water and resistant to acids, 
alkalis, organic solvents, and moderate heat. 
Appendix G—II-A—A-c. Laboratory furniture 
is sturdy. Spaces between benches, cabinets, 
and equipment are accessible for cleaning. 
Appendix G—ll-A—t—d. Each laboratory 
contains a sink for hand-washing. 
Appendix C-H-A—t—e. If the laboratory has 
windows that open, they are fitted with fly 
screens. 
g. The following language would be 
substituted for Appendix G— II— B, P2 
Level: 
Appendix G-Il-B. Biosafety Level 2 (BL2). 
M- 
Appendix G-IL-B- 1. Standard 
Microbiological Practices. 
Appendix G-II-B-l-a. Access to the 
laboratory is limited or restricted by the 
laboratory director when work with 
organisms containing recombinant DNA 
molecules is in progress. 
Appendix G-Il-B— 1-b. Work surfaces are 
decontaminated at least once a day and after 
any spill of viable material. 
Appendix G-ll-B-l-c. All contaminated 
liquid or solid wastes are decontaminated 
before disposal. 
Appendix G-ll-B-l-d. Mechanical 
pipetting devices are used; mouth pipetting is 
prohibited. 
Appendix G-Il-B- 1-c. Eating, drinking, 
smoking, and applying cosmetics are not 
permitted in the work area. Food may be 
stored in cabinets or refrigerators designated 
and used for this purpose only 
Appendix G-II-B-l-f. Persons wash their 
hands after handling materials involving 
organisms containing recombinant DNA 
molecules, and animals, and when they leave 
the laboratory. 
Appendix G—ll- B-l-g. All procedures are 
performed carefully to minimize the creution 
of aerosols. 
Appendix C-ll-B-I-b. Experiments of 
lesser biohazard potential can be carried out 
concurrently in carefully demarcated areas of 
the seme laboratory. 
Appendix G-II-B-2. Special Practices. 
Appendix G-II-B-2-a. Contaminated 
materials that are to be decontaminated at a 
site away from the laboratory are placed in a 
durable leakproof container which is closed 
before being removed from the laboratory. 
Appendix G-ll-B-2-b. The laboratory 
director limits access! to the laboratory. The 
director has the final responsibility for 
assessing each circumstance and determining 
who may enter or work in the laboratory. 
Appendix G-II-B-2-c. The laboratory 
director establishes policies and procedures 
whereby only persons who have been 
advised of the potential hazard and meet any 
specific entry requirements [e.g., 
immunization) enter the laboratory or animal 
rooms. 
Appendix G-ll-B-2-d. When the organisms 
containing recombinant DNA molecules in 
use in the laboratory require special 
provisions for entry (e.g., vaccination), a 
hazard warning sign, incorporating the 
universal biohazard symbol, is posted on the 
access door to the laboratory work area. The 
hazard warning sign identifies the agent, lists 
the name and telephone number of the 
laboratory director or other responsible 
person(s). and indicates the special 
requirement(s) for entering the laboratory. 
Appendix G-II-B-2-e. An insect and 
rodent control program is in effect. 
Appendix G-II-B-2-f. Laboratory coats, 
gowns, smocks, or uniforms are worn while 
in the laboratory. Before leaving the 
laboratory for nonlaboratory areas (e.g.. 
cafeteria, library, administrative offices), this 
protective clothing is removed and left in the 
laboratory or covered with a clean coat not 
used in the laboratory. 
Appendix G-II-B-2-g. Animals not 
involved in the work being performed are not 
permitted in the laboratory. 
Appendix G-II-B-2-h. Special care is taken 
to avoid skin contamination with organisms 
containing recombinant DNA molecules; 
gloves should be worn when handling 
experimented animals and when skin contact 
with the agent is unavoidable. 
Appendix G-Il-B-2-i. All wastes from 
laboratories and animal rooms are 
appropriately decontaminated before 
disposal. 
Appendix G-H-B-2-j. Hypodermic needles 
and syringes are used only for parenteral 
injection and aspiration of fluids from 
laboratory animals and diaphragm bottles. 
Only needielocking syringes or disposable 
syringe-needle units |i.e., needle is integral to 
the syringe) are used for (tie injection or 
aspiration of fluids containing organisms that 
contain recombinant DNA molecules. 
Extreme caution should be used when 
handling needles and syringes to uvoid 
hiitoinoculation and the generation of 
aerosols during use and disposal. Needles 
should not be bent, sheared, replaced in the 
sheath or guard or removed from the syringe 
following use. The needle and syringe should 
be promptly placed in a puncture resistant 
container and decontaminated, preferably by- 
autoclaving, before discard or reuse. 
Appendix G-ll-B-2-k. Spills and accidents 
which result in overt exposures to organisms 
containing recombinant DNA molecules are 
immediately reported to the laboratory 
director. Medical evaluation, surveillance, 
and treatment are provided as appropriate 
and written records are maintained. 
Appendix G-II-B-2-1. When appropriate, 
considering the agent(s) handled, baseline 
serum samples for laboratory and other at- 
risk personnel are collected and stored. 
Additional serum specimens may be 
collected periodically, depending on the 
agents handled or the function of the facility. 
Appendix G-ll-B-2-m. A biosafety manual 
is prepared or adopted. Personnel are 
advised of special hazards and are required 
to read instructions on practices and 
procedures and to follow them. 
Appendix G-II-B-3. Containment 
Equipment. 
Appendix G-U-B-3-a. Biological safety 
cabinets (Class I or II) (See Appendix G — HI- 
12) or other appropriate personal protective 
or physical containment devices are used 
whenever 
Appendix G-II-B-3-a-(l). Procedures with 
a high potential for creating aerosols are 
conducted [15]. These may include 
centrifuging, grinding, blending, vigorous 
shaking or mixing, sonic disruption, opening 
containers of materials whose internal 
pressures may be different from ambient 
pressures, inoculating animals intranasally, 
and harvesting infected tissues from animals 
or eggs. 
Appendix G-II-B-d-a-f2). High 
concentrations or large volumes of organisms 
containing recombinant DNA molecules are 
used. Such materials may be centrifuged in 
the open laboratory if sealed heads or 
centrifuge safety cups are used and if tney 
are opened only in a biological safety 
cabinet. 
Appendix G-II-B-4. Laboratory Facilities. 
Appendix G-Il-B-A-a. The laboratory is 
designed so that it can be easily cleaned. 
Appendix G-H-B-4-b. Bench tops are 
Impervious to water and resistant to acids, 
alkalis, organic solvents, and moderate heat. 
Appendix G-Il-B-4—c. Laboratory furniture 
is sturdy and spaces between benches, 
cabinets, and equipment are accessible for 
cleaning. 
Appendix C—Il-B-i-d. Each laboratory 
contains a sink for handwashing. 
Appendix G-II-B-4—e. If the laboratory has 
windows that open, they are fitted with fly 
screens. 
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