15 
"B. Vectors and Method of Introduction. 
"!• Describe the cloned DNA segment and its expression in the new host. 
"2. Describe the method(s) by vhich the proposed DNA vector will be or 
has been constructed . Diagrams are very helpful and rray be neces- 
sary for adeguate understanding of the construct . Explain the 
advantages (and disadvantage(s) , if appropriate) of your vectors, 
if other candidate vectors could be considered. 
"3. If microorganisms are used to introduce vectors or are vectors 
themselves , indicate how they compare with wild-type strains. If 
disabled pathogens are used to transmit the vector, indicate fac- 
tors that will most likely prevent these microorganisms frcm 
regaining or acguiring pathogenic potential. If the vector is 
likely to survive independently of the desired host(s), refer to 
this possibility and provide any available data to assess the 
probability of transfer to other organisms. 
"4. If microorganisms are used to introduce vectors, the absence of 
these microorganisms in the plants to be released in the field 
should be documented . 
"C. Characteristics and Monitoring of Genetically Engineered and Control 
Plants . 
"1. Provide data frcm greenhouse and/or growth chamber studies to sup- 
port prospective field studies. Include morphological data for at 
least two generations of plants if feasible. Supply any molecular 
or physiological data, especially as applicable to the trait (s) 
under consideration. 
"Specify plant monitoring procedures, frequency, and types of data 
obtained . 
"2. Field plots should meet the criteria specified in Appendix L-II-D: 
"Appendix L-II-D. Plants are grown in controlled access fields 
under specified conditions appropriate for the plant under study 
and the geographical location. Such conditions should include 
provisions for using good cultural and pest control practices, for 
physical isolation frcm plants of the same species outside of the 
experimental plot in accordance with pollination characteristics 
of the species, and for further preventing plants containing 
recombinant DNA frcm becoming established in the environment. 
Review by the IBC should include an appraisal by scientists 
knowledgeable of the crap, its production practices, and the local 
geographical conditions. Procedures for assessing alterations in 
and the spread of organisms containing recombinant ENA must be 
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