Attachment II - Page 1 
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Points to Consider for Submission Under Appendix L. 
Appendix L of the Guidelines specifies conditions under which certain 
plants may be approved for "release into the environment" including field 
tests. Experiments in this category cannot be initiated without submission 
of relevant information on the proposed experiments to NIH, review by the 
RAC Plant Working Group, and specific approval by NIH. 
Ihe proposal should include a statement of objectives and a description of 
materials and methods, including methodology for monitoring the experiments, 
and expected results. A summary of relevant preliminary results should 
accompany the proposal. Information to be submitted should include but 
not be limited to: 
A. Description of Plant Materials. 
Give common and scientific names of plants. Identify the specific 
cultivars or genetic lines to be used. Include information on the 
relative homogeneity of the plant cultivars or lines and specific 
genetic markers they are known to possess. 
B. Vectors and Method of Introduction. 
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1. Describe the cloned DNA segment and its expression in the new 
host. 
2. Describe the method (s) by which the proposed DNA vector will be or 
has been constructed. Diagrams are very helpful and may be necessary 
for adequate understanding of the construct. Explain the advantages 
(and disadvantage (s ) , if appropriate) of your vectors, if other 
candidate vectors could be considered. 
3. If microorganisms are used to introduce vectors or are vectors 
themselves, indicate how they compare with wild-type strains. If 
disabled pathogens are used to transmit the vector, indicate factors 
that will most likely prevent these microorganisms from regaining 
or acquiring pathogenic potential. If the vector is likely to 
survive independently of the desired host(s), refer to this possibility, 
and provide any available data to assess the probability of 
transfer to other organisms. 
4. If microorganisms are used to introduce vectors, the absence of 
these microorganisms in the plants to be released in the field 
should be documented. 
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