Attachment II - Page 2 
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C. Characteristics and Monitoring of Genetically Engineered and Control 
Plants. 
1. Provide data from greenhouse and/or growth chamber studies to 
support prospective field studies. Include rrorphological data 
for at least two generations of plants as appropriate. Supply any 
molecular or physiological data, especially as applicable to the 
trait(s) under consideration. 
Specify plant monitoring procedures, frequency, and types of 
data obtained. 
2. Field plots should meet the criteria specified in Appendix L-II-D: 
"Appendix L-II-D. Plants are grown in controlled access fields 
under specified conditions appropriate for the plant under study 
and the geographical location. Such conditions should include 
provisions for using good cultural and pest control practices, 
for physical isolation from plants of the same species outside 
of the experimental plot in accordance with pollination charac- 
teristics of the species, and for further preventing plants 
containing recombinant CNA from becoming established in the 
environment. Review by the IBC should include an appraisal by 
scientists knowledgeable of the crop, its production practices, 
and the local geographical conditions. Procedures for assessing 
alterations in and the spread of organisms containing recombinant 
DMA must be developed. ''The results of the outlined tests must 
be submitted for review by the IBC. Copies must also be submitted 
to the Plant Working Group of the RAC." 
Supporting data should include the following: 
a. total area; 
b. geographical location(s): where, how many locations; 
c. plot design: for example, replication, row spacing, 
nature of border rows; 
d. specify plant monitoring procedures: frequency; 
types of data to be obtained, including leaf, 
seed, fruit, or root characteristics; disease, 
insect and other animal population monitoring as 
appropriate . 
e. specify techniques for monitoring the vector and/or altered 
DNA; and 
f. specify access and security measures." 
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