appropriate, the large-scale containment recommendations of 
the NIH should be used (45 FR 24968). 
Appendix C-II, C-III and C-IV 
Exceptions 
Large scale experiments (i.e., more than 10 liters of 
culture) require IBC notice simultaneously with the 
lDin§tlgn_gf_experimeny_where_]BC r apprgyed.gractices_and_an 
IBC-approved Pl-LS containment facility will be used. Where 
these conditions are not satisfied, refer to Part III-B-5*. 
Our second comment pertains to the Recommendations for 
Physical Containment for Large-Scale Uses of Viable Organisms 
Containing Recombinant DNA Molecules (45 FR 24968-24971). 
With respect to the physical containment recommendations for 
large-scale cultivation of recombinant DNA-contai ni ng 
organisms, we believe there are two areas of Part VII which 
require clarification. 
First, we feel that the language in Part VII-B-1, VII-C-1 and 
VII-D-1 is ambiguous and suggest that it be changed to read 
as follows: 
"Cultures of viable organisms containing recombinant DNA 
molecules shall be handled in a closed system used_for_the 
propagation, growth and processing of cuHures , _other_pri_mary 
containment equipment or other appropri_ate_method _of 
containment approved by the IBC* which is designed to reduce 
the potential for escape of viable organisms...". 
* indicates proposed changes in wording. 
[ 595 ] 
