246 
East Coast Fever 
Our results are not given in terms of weight of haemoglobin, but 
of pressure of liberated oxygen in the manometer: 
Cow 11 
November 
26 
26 mm. 
December 
8 
23 mm. 
99 
9 
23 mm. 
99 
16 
24 mm. 
Cow III 
February 
4 
15 mm. 
99 
15 
16 mm. 
Cow IV 
February 
24 
16 mm. 
March 
10 
16-5 mm. 
These figures show that the amount of haemoglobin in the blood 
undergoes no appreciable change during the course of the disease and 
they confirm the other evidence that no unusual blood destruction 
occurs. 
3. The Specific Gravity. 
Estimations were made of the specific gravity of the blood because 
it was thought that an actual decrease of the red corpuscles was perhaps 
being compensated for by an increased specific gravity. 
The method used was the “jar method.” A series of jars held 
mixtures of xylol and absolute alcohol in varying proportions so as to 
give fluids of a series of different specific gravity, these being calculated 
by using specific gravity beads. Into each of these jars a drop of the 
blood to be estimated was placed, and the fluid in which the blood 
neither rose nor sank gave the correct specific gravity of the blood. 
Cow 11 
December 1 
Sp. gr. 
1057-5 
„ 8 
1055-5 
„ 9 
1053-5 
Cow III 
February 5 
1058 
„ 16 
1057-5 
Cow IV 
February 25 
1055-5 
March 7 
1053 
It will thus be seen that far from the specific gravity going up, it 
actually shows a slight decrease. 
4. The White Cells. 
The white cells were counted by means of Thoma-Zeiss’s haemocyto- 
meter, using Toisson’s fluid as a diluent. As a rule 375 squares were 
