87 
Figure VIM. Example of a vertical density profile with calculated pycnocline boundaries 
and observed dissolved oxygen concentrations with depth. Monitored water column 
density and observed dissolved oxygen concentrations with depth are illustrated with the 
upper (dashed line) and lower (dotted line) pycnocline depths overlaid for station CB4.3 in 
the middle Chesapeake Bay mainstem on June 10, 1986. 
LITERATURE CITED 
Brainerd, K. E. and M. C. Gregg. 1995. Surfaced mixed and mixing layer depths. Deep-Sea 
Research 42: 1521-1543 
Chesapeake Bay Program. 1996. Recommended Guidelines for Sampling and Analyses in the 
Chesapeake Bay Monitoring Program. EPA 903-R-96-006. CBP/TRS 148/96. Chesapeake 
Bay Program Office, Annapolis, Maryland. 
Culver, M. E. and M. J. Perry. 1999. The response of photosynthetic absorption coefficients 
to irradiance in culture and in tidally mixed estuarine waters. Limnology> and Oceanography 
44: 24-36. 
Fisher, Tom. 2003. Personal communication/unpublished manuscript. University of Mary¬ 
land Center for Environmental Science, Horn Point Laboratory, Cambridge, Maryland. 
Larsson, U., S. Hajdu, J. Waive, and R. Elmgren. 2001. Baltic Sea nitrogen fixation estimated 
from the summer increase in upper mixed layer total nitrogen. Limnology ; and Oceanography 
46: 811-820. 
U.S. Environmental Protection Agency. 2003a. Ambient Water Quality; Criteria for Dissolved 
Oxygen, Water Clarity’ and Chlorophyll a far the Chesapeake Bay and Its Tidal Tributaries. 
EPA 903-R-03-002. Region III Chesapeake Bay Program Office. Annapolis, Maryland. 
U.S. Environmental Protection Agency. 2003b. Technical Support Document for Identifica¬ 
tion of Chesapeake Bay Designated Uses and Attainability. EPA 903-R-03-004. Region III 
Chesapeake Bay Program Office, Annapolis, Maryland. 
chapter vii 
Upper and Lower Pycnocline Boundary Delineation Methodology 
