14 
rimaculatus above the bayou: 2 A. quadrimaculatus out of 28 An¬ 
opheles. The “backwater” of the creek was practically barren, and 
no Anopheles larvae of any kind were found except at the upper end. 
The bayou yielded both species, but with A. qwadrimaculotus heavily 
predominating. 1 Some bights on the shore line of the pond just 
above this bayou,- in which hammocks had lodged, showed the same 
picture. 2 In general a somewhat larger proportion of A. punctip- 
ennis were found in the pool water than at these places, but except in 
Cedar Creek we practically did not find, in the vicinity of the pond, 
A. quadrimaculatus mosquitoes in live water of streams, in marshes, 
or in small pools. They were found only in the pond itself and in 
pools which were of some size, as in a large pool up Spring Creek and 
a concrete tank at Talladega Springs. Our records show over 1,600 
imagos developed during the August and September survey in addi¬ 
tion to 600 or 800 A. punctipennis sent to the laboratory. These are 
enough to base a judgment on. 
In the upper part of the backwater of Cedar Creek, larvae were 
found in leaf drifts. No Anopheles larvae were found in the lower 
half mile of backwater and practically none in the bayou of this 
creek, except on the north side one-quarter mile from the mouth. 
A. crucians were found here in bait holes, 3 as were the other two 
species. 
Factors determining breeding .—The breeding in the pond at this 
time was decidedly irregular in amount. The factors which seem 
to have determined the breeding were such as give protection to the 
larvae and possibly furnish food for them. They were not found 
where subject to wave action nor where fish could readily get at 
them. On the other hand, where a collection of drift in front of a 
bight protected it from wave action and sheltered a mass of floatage 
with living algae in it, they were frequently found. In the pond they 
were found more often in water over 2‘ feet deep than in shoal 
water. Possibly this is because of the presence of fish. They were 
rarely found except in floatage, usually fine floatage with algae or 
leaves; heavy drift did not seem to be sufficient. On the gently slop¬ 
ing edges of the pond they were practically absent. They were much 
more common against a steep bank, if material to shelter them was 
lodged a little way from it, as was common in bights with steep 
banks. The gently sloping edges were free from floatage, since the 
rise of the water level, small as it was, and wave motion had stranded 
the floatage ashore. 
Why larvae were so rarely found in the backwater of creeks is 
unknown. This zone, except the upper part, was usually barren. 
J Two A. punctipennis to 14 A. quadrimaculatus is one record: 21 to 120 is another. 
2 A. punctipennis 0, A. quadrimaculatus 24, is one entry; A. punctipennis 2. A. quadri- 
maeulatus 60, another; and A. punctipennis 8, A. quadrimaculatus 64. yet another. 
3 These are holes made by digging for angleworms They were tilled with seepage water. 
