21 
during the overwintering period. A mosquito which may enter upon the hiber¬ 
nating stage in an infective state and which confines its biting to animals 
housed for the winter may clear its salivary glands of sporozoites before it 
again bites a human being. 
A few notes concerning conditions of hibernation at Talladega Springs were 
made in the course of collecting specimens during January 8-9, 1916. An 
examination was made of five streams and one cement reservoir from which 
numerous anopheline larvae had been taken in the course of a mosquito survey 
in September and October, 1915, by Epidemiologist Griffitts of the service. 
Several Culex egg boats and cast puparia were collected, but neither eggs nor 
larvae of Anopheles were seen. Two trained observers from this locality re¬ 
ported that developed larvae and pupae of Anopheles were seen in protected 
streams in November, and as late as the last week of December, 1915. 
Specimens of Anopheles punctipennis and quadrimacnlatus were caught, on 
the first evening of the investigation (Jan. 8) while biting cattle. The tempera¬ 
ture at this time was 21° C. General infestation at this place had been seen a 
few days previously when an unusual rise in temperature had been recorded. 
Some idea of at least the maximum temperature during the survey at this 
place can he gained from the following table: 
Table No. III. 
Date. 
Temper¬ 
ature. 
, Time of 
day re- 
I corded. 
Date. 
1 Temper¬ 
ature. 
i Time of 
1 dayre- 
! corded. 
Jan. 3,1916. 
° c. 
15.0 
5.30p.m. | 
8 p.m. 
Jan. 7,1916. 
° C. 
20.0 
7 a. m. 
12.0 
20.0 
j 12 m. 
Jan. 4,1916. 
8.0 
7 a. m. 
10.0 
8.30p.m. 
15.0 
12 a. m. 
Jan. 8,1916. 
8.0 
7 a. m. 
Jan. 5,1916. 
14.5 
13.5 
8 p.m. 
7 a. m. 
14.0 
9.5 
12 m. 
6 p. m. 
21.0 
7 p. m. 
Jan. 9,1916. 
7.5 
7 a. m. 
Jan. 6,1916. 
20.5 
7 a. m. 
13.5 
12 m. 
21.0 
6 p.m. 
The following table enumerates the distribution of the 392 anophe- 
lines collected in this locality, January 3 to 9, 1916: 
Table No. IV.— Giving the distribution of Anopheles found in Talladega 
Springs, Ala. 
Number of mos¬ 
quitoes. 
Location. 
A.punc- -4-?™?- 
tipknnis. nm t a u c s ula - 
W ithin dwellings. 
Under buildings. 
Mule and horse stables 
Cattle stables. 
Biting cattle in open.. 
Harness room. 
Feed house.. 
Privies. 
Total (392). 
27 
81 
159 
6 
4 
78 
26 
388 j 4 
Dissections were made of 371 specimens collected. Xo organisms 
resembling Plasmodium were found. It is interesting to note in this 
connection that mosquitoes which have been interrupted in their liiber- 
