16 
It was established beyond a reasonable doubt that the new spring 
brood was not likely to have received the initial infections of malaria. 
Certainly the females of the winter brood had greater opportunities 
to obtain human blood to the extent of at least two weeks antedating 
the appearance of the spring brood. During May 1—10 a special 
effort was made to collect specimens of the spring brood attacking 
persons in houses. Many houses of both whites and negroes were 
visited during this period and the writer exposed himself in an un¬ 
screened plantation house in order to collect specimens at first hand. 
In all this, while not a specimen of male Anopheles was seen, the 
females dissected were either fecund with developed eggs or supplied 
with masses of fat cells, indicative of matured mosquitoes. 
In this search it was particularly noted that the mosquitoes preva¬ 
lent observed their natural habits of biting and in the usual house 
infestation. In one morning’s collecting 60 specimens of Anopheles 
quadrimaculatus were taken from a negro cabin located on the edge 
of a swampy woods. The residents, nearly all of whom had been 
previously infected with malaria, complained that the mosquitoes 
were so bad at nights as to interfere with sleep and that it was found 
necessary to resort to smudging. In the kitchen of this dwelling 
the smoke was very dense; here no mosquitoes were found. 
As in previous collecting, no male Anopheles were observed, and 
many of the females were gravid and laid fertile eggs when kept 
in containers a few days previous to their dissection in the laboratory. 
In one negro cabin typical epidemiological factors were presented. 
The house, of two living rooms, was occupied by 12 persons, 1 at 
least had crescents in her blood and at least 5 of the remainder 
harbored asexual parasites. The house was 300 meters distant from 
the neighbor’s on the edge of a clearing in the woods, which in 
winter is ordinarily very swampy. The nearest trees were 25 meters 
away. The cabin and adjoining fowl roost yielded 145 specimens of 
A. quadrimacvlatus. More than 100 were collected in a single room; 
36 of them from behind one of the beds in which a child was then 
sleeping. A ladder was used to obtain the mosquitoes from the walls 
and ceiling. The walls were constructed of rough pine boarding 
and the ceiling of close-fitting finished lumber. Xo whitewash, as 
it is commonly seen, was used in this room. The mosquitoes were, 
for the most part, resting on objects of furniture, particularly upon 
the three bedsteads in the corners. A black umbrella alongside the 
bed yielded 3 mosquitoes, the headboard of a bed yielded 7, a spider 
web near the ceiling yielded 6, and the fireplace within the chimney 
yielded 12 mosquitoes. Many of these mosquitoes were seen upon 
the ceiling after being disturbed below. Several specimens were 
taken from the bed net which covered the sleeping child. The fowl 
roost yielded 35, and 9 were taken from beneath the cabin. The dis- 
