15 
On April 11 there was noted for the first time a perceptible dis¬ 
appearance of Anopheles from the woods, and coincidentally the re¬ 
sumption of normal parasitism, biting in the evening rather than in 
daylight. This was ascertained by a trip on horseback covering 11 
miles through the woods and swamps. The horses were rested at 
frequent intervals, especially in regions where the greatest number of 
mosquitoes were collected previously. Only a few specimens of 
Culex and Simulium were captured. 
The normal nocturnal biting habit was brought to the attention of 
the writer by the manager of Myers plantation along Bolivar Lake, 
a few miles north of Scott. Complaints of biting at night, and the 
presence of numerous anophelines in the corner of the screened house 
gallery, were definite proofs. This occurred during the last week of 
April and on May 2. It was assumed by the manager that the stiff 
breezes of recent occurrence drove mosquitoes which emerged from 
the waters of Kentucky Shoot, on the opposite side of Lake Bolivar, 
to the plantation manager’s house, a distance of 400 meters. 
A survey was therefore instituted to ascertain, if possible, the 
source of the Anopheles infestation on Myers plantation. A day was 
occupied in a small skiff, inspecting Lake Bolivar in the region of 
Kentucky Shoot. It was found that the latter was a shallow bayou 
10 meters in width at its maximum, extending 500 meters through 
Isole plantation. Dense cypress extended on both sides of the 
stream, the head of which formed a stagnant swamp draining a 
ditch from an adjoining cotton field. The open woods along the 
banks of the shoot formed a pasturage for animals, seen grazing at 
the time of our survey. Near the mouth of the shoot, in the matted 
algae and debris, a few Aedes larvae were found. This form only was 
encountered for a distance of 75-100 meters, then larvae of Anopheles 
predominated for a distance of 60 meters. An average of one speci¬ 
men of Anopheles larva to two dipperfuls of water was collected. 
During the entire period of the inspection, Aedes bit us freely, but 
not a single instance of Anopheles biting was observed along the 
shoot or in the woods near by. While in the pasture land a few 
Anopheles were caught biting us and a grazing pony. 
It was concluded from the two days’ survey that some source 
other than Kentucky shoot was responsible for the Anopheles in¬ 
festation across the lake at Myers plantation. 
In the collection of 308 Anopheles made at Myers plantation at 
this time (on or before May 2) only specimens of females were 
caught, and these, in the majority of instances, showed ovaries dis¬ 
tended with eggs. It was concluded, therefore, that the spring brood 
was not in existence before May 2, and that the mosquitoes en¬ 
countered were probably, without exception, from the overwintered 
brood. 
