Stations— 
There were a total of six stations. The control station was 
located at least 1-1/2 km upstream from the Tenneco plant. This 
station was in a small tributary to Morgan Creek but the same one 
which carries the overflow from the discharge pond of the Tenneco 
Pond to Morgan Creek. 
Tenneco Pond located in the Tenneco discharge pond about 6 m 
away from the standpipe which is the point farthest away 
from the plant's point of discharge. Depth at this station 
was about 0.6 m. 
Tenneco Downstream—in the receiving stream from the Tenneco 
Pond about 350 m downstream from the pond. Depth at this 
station was 0.5 m maximum. No tidal influence was noted. 
Campbell Upstream—in Morgan Creek near the middle discharge 
pipe of the Campbell leaching fields in 0.8 m depth at low 
tide. Due to tidal amplitude, this station could only be 
checked once a day as at high tide the depth became 1.6 m 
or more. 
Campbell Downstream—also in Morgan Creek about 500 m from 
station upstream and located just upstream from Campbell's 
most downstream discharge. Tidal difference here was also 
more than 0.6 m, which often limited mortality checks to 
once a day. 
Sewage Plant—was located in Radcliffe Creek about 6 m down¬ 
stream from the sewage outfall. Tidal difference varied 
depth from 0.5 m to well over 1 m, which allowed only 
one inspection per day. 
Procedures-- 
Experimental species for this investigation were the golden 
shiner, Notemigonas crysoleucas and the crayfish, Procambarus 
acutis acutis . These species were chosen because they are 
native to the area, are easily available, and easily acclimated 
to new conditions and represent animals which are neither overly 
delicate nor particularly pollution resistant forms. Golden 
shiners were obtained from Green Valley Minnow Farms, Brogue, 
Pennsylvania. Crayfish were obtained by seining various creeks, 
ditches, and rivers on the Eastern Shore. All animals were held 
for a minimum of 2 weeks in sand-filtered Solomons well water. 
Temperatures were adjusted to those that were expected to be 
encountered in the field. Both species were fed Purina Trout 
Chow daily. Transportation to and from the laboratory was in a 
1400 1 tank aerated using tanks of compressed air. 
Test animals were contained in cages of 30.5 cm x 30.5 cm x 
91.4 cm with a 13 mm rebar frame covered with 3.2 mm mesh nylon 
netting. 
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