The experiment lasted 96 hours. One cage with 30 golden 
shiners and one cage with 15 crayfish were put overboard at each 
station. Where possible, the cages were checked twice daily and 
dead animals were removed and preserved for future autopsy. 
Once a day the animals were fed Purina Trout Chow®. 
All survivors of the 96-hour experimental period were pre¬ 
served in Davidson's preservative for later autopsy. 
Hydrographic data, recorded at every visit, were temperature, 
salinity, dissolved oxygen and residual chlorine (see Table 2 for 
these data). 
Autopsy of Specimens-- 
The autopsy of golden shiners was divided into two parts-- 
external and internal features. External features included 
standard length, examination for damage such as broken, absent 
of excessive mucus covering; missing or deformed scales, abra¬ 
sions on body and/or fins; and afflictions such as fungus, dis¬ 
colorations, cysts and parasites. Internal autopsy examined 
general appearance, color, damage and foreign material on or in 
the buccal cavity, gills and gill arches; texture, size, color, 
content and abnormalities of stomach, internal and external 
intestinal lining, liver, gasbladder, gonads, spleen and adipose 
tissue. Any observations that did not fit into the prepared 
autopsy sheet were recorded under item "Other." All specimens 
were individually wrapped in a bag of cheesecloth containing an 
identifying tag and were placed in fresh Davidson's solution. 
Cages containing crayfish were checked at the same time as 
the cages with golden shiners. At that time dead crayfish were 
removed and preserved in Davidson's solution. All surviving 
crayfish were preserved at the end of the 96-hour experimental 
period. 
Autopsy of crayfish examined both external and internal 
features. External features such as injury, regeneration to ap¬ 
pendages and body, hardness of cephalothorax, color, affliction 
with fungus, discoloration, and bacterial infection were recorded. 
Internal features such as condition and foreign material in 
the gills and gill chamber, texture and content of cardiac and 
pyloric stomach, condition and appearance of heart, hepatopan- 
creas and gonads were recorded. 
After autopsy the specimens were wrapped individually in a 
cheesecloth bag that contained an identification tag and placed 
in fresh Davidson's preservative. 
Autopsy data were examined for correlations using contingency 
analysis with chi-square tests. 
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