62 
September 14, at 4 p. m., Cruz complained of feeling chilly and 
had frontal headache. The chilly sensation lasted for several hours 
and the headache became so severe that he was given ice caps to his 
forehead for the relief of this symptom. The conjunctive became 
injected; the gums turgid and red. 
The case continued clinically with typical symptoms of yellow 
fever. 
On September 15, at 8 a. m., there was a slight icterus of the 
eyes; at 10 a. m. there was marked vomiting; at 7 p. m. the gums were 
yery much swollen. Urine contained no albumin. 
September 16: Eyes injected and yellowish, skin jaundiced; no 
albumin. 
On September 17, the third day of the disease, albumin first 
appeared in the urine and Avas found daily until September 25, after 
Avhich examination was discontinued. 
On September 15, at 8 a. m., 16 hours after the onset of the disease 
80 cc. of blood Avere drawn into a sterile flask by means of an aspirat¬ 
ing needle from the median basilic vein of his left arm. 
The flask was set aside in the loAver part of the ice chest, at a tem¬ 
perature of from 16° to 19° C., for fh T e hours in order to allow the 
blood to coagulate. 
Thirty-five cubic centimeters of the clear serum were then draAvn 
off, and to it was added an equal volume of physiological salt solu¬ 
tion. 
The mixture was transferred with all due precautions to the inside 
of a Pasteur-Chamberland B bougie and filtered from Avithin out¬ 
ward by means of vacuum, which Avas applied to the outer surface 
of the filter in a reverse manner to that shown in fig. 2, omitting 
the paraffin cup. 
It required one hour to obtain 13.5 cc. of filtrate, which was used 
for the'injection of three nonimmunes, Bonifacio Orea, German 
Kamos, and Guadalupe Gomez. 
Orea and Ramos each received 5 cc. of the filtrate, injected intra¬ 
venously into one of the veins of the arm. As the same was diluted 
with an equal volume of salt solution, each man received 2.5 cc. of 
the original blood serum. 
Gomez received 3.5 cc. of the mixture, representing 1.75 cc. of the 
original serum. 
These injections were made at 4 p. m. September 15, which was 
just eight hours after the blood had been drawn from Cruz, the 
blood having kept five hours at a temperature of 16° to 19° C. and 
three hours at room temperature. 
