71 
Peripneumonia. —Peripneumonia ° of cattle is the only filterable 
virus which has so far given a visible growth on artificial media. 
Collodion sacs were filled with Martin’s peptone bouillon, to which 
was added a little serum of the rabbit or cow in the proportion of 1:20. 
The sac was then inoculated with peripneumonia and placed in the 
peritoneal cavity of rabbits and cows for fifteen to twenty days. 
The fluid became turbid and in it, under a magnification of 2,000 
diameters, could be seen the most extremely small, moving, strongly 
refractile points. In a series of subcultures made from such a growth 
the last of the series was virulent. 
The colonies on agar mixed with bouillon-serum, were transpar¬ 
ent, small, and made up of exceedingly fine particles whose form it 
was impossible to determine. The microbe of this disease was made 
to pass the Berkefeld and Chamberland F filters, but when an 
albuminous diluting liquid was used, it could not be made to pass 
either. * * & 
Foot-and-mouth disease .—Loeffler and Frosch c state that lymph 
was taken from the blebs of calves suffering with this affection, 
diluted with 35 parts of water, and then passed through a filter 
candle. The filtrate, in amounts which correspond to one-tenth to 
one-fortieth cubic centimeter of the original lymph, when injected 
into calves caused them to sicken in two days, the same as the con¬ 
trol animals into which were injected equal amounts of unfiltered 
fluid. 
McFadyean says that foot-and-mouth disease passes the Berkefeld 
filter when in watery suspension, but is arrested when in an albumi¬ 
nous fluid. 
Nocard d says that aphthous fever passes through Berkefield, Cham¬ 
berland, and Ivitasato filters. 
South African horse sickness. —Nocard® succeeded in passing the 
virus of this disease through a Berkefeld filter only. 
McFadyean e reports that pure blood taken from an animal sick 
with the disease was passed through the Berkefeld filter under a 
pressure of 26 inches of mercury and the filtrate, when inoculated 
into a horse, produced the disease. 
When the blood serum was diluted with four parts of water and 
a Nocard, Roux, Borrel, Salimbeni et Dujardin-Beaumetz: Le microbe de la 
peripneumonie. Ann. de PInst. Pasteur, vol. 12, 1898, p. 240, etc. 
6 Nocard, Roux, and Dujardin-Beaumetz: Etudes sur la peripneumonie. Re- 
cuil de med. vet., 1899, 8e. serie, Oct. 26, 1899, p. 441. 
cLoeffler and Frosch: Bericht der kommission zur erforschung der maul 
und klauenseuche bei dem Inst. f. Infek.-krank. in Berlin. Centbl. f. bakt. 
u. infek., 1898, bd. 23. 
d Nocard: La “ horse sickness ” ou “ maladie des chevaux de l’Afrique du 
Sud. Bull, de le soc. centr. de med. vet., n. s., vol. 19, 1901, p. 37. 
«Journ. comp. path, and therap., 1900, XIII. 
