77 
capable of reproducing the symptoms of the disease when injected 
into the veins of other men. 
We are not unmindful of the fact that chemical substances derived 
from the hemoglobulin or other proteids in the blood may be toxic, 
and we are of course familiar with the work of Gauldi, Montesano, 
Mannaberg, Celli, and others, who failed to demonstrate a pyrogenic 
toxin in malarial blood from similar experiments. The length of 
time the blood was exposed to the air between the time it was drawn 
from the malarial patient until it was injected into the person experi¬ 
mented upon may account for the discrepancies in results. The time 
the blood is drawn in relation to the paroxysm and many other factors 
should also be taken into account. 
Mannaberg® drew blood during the attack in a case of ordinary 
tertian malaria. He centrifugalized it and injected the clear serum 
subcutaneously into two healthy people. 
One received 1 cc. of serum at 4 p. m., wdien his temperature was 
36.7° C. The temperature at 4.30 p. m. was 37° and at 6 o’clock 36°. 
The other patient was given 0.7 cc. of the serum and his temper¬ 
ature rose within fifteen minutes after the injection from 36.5° to 
37.6° C. 
Celli * * & took during the cold stage a small quantity of blood from 
each of many malarial patients. 
Young children were inoculated with 50 cc. of the serum sub¬ 
cutaneously and 50 cc. intravenously. 
Another child was given the concentrated serum remaining after ’ 
treating 260 cc. of serum in a vacuum apparatus at low temperature. 
The child was injected intravenously and subcutaneously. 
From a hemorrhage in a case of severe comatose pernicious malaria 
25 cc. of serum were obtained and injected into another patient. 
None of the patients into whom the serum was injected showed 
pyrexia. There was in several instances, however, a slight rise of 
temperature which the experimenter says may occur after the injec¬ 
tion of normal serum. 
Kievel and Behrens c studied a sarcosporidium of the llama. They 
removed ten of the sacks and ground them up with physiological salt 
solution in a mortar, and injected 2 cc. of the fluid subcutaneously 
into a rabbit. After seven hours the rabbit died. The autopsy 
revealed nothing unusual. 
a Mannaberg, Julius: Die malaria krankheiten. Notknagel’s Specielle Path- 
ologie und Tkerapie, Bd. 2, 1899. 
& Celli, Angelo: Malaria. Transl. by J. J. Eyre. Longmans, Green & Co., 
New York and London, 1900. 
c Rievel and Behrens: Beitriige zur Kenntnis der Sarcosporidien und deren 
Enzyme. Centralblatt fur bakt. u. parasit. (orig.). Bd. 35, no. 3, s. 341. 
