79 
The blood was immediately put into the ice chest, the temperature 
of which registered between 16° and 19° C. Clotting took place 
rapidly. The red cells settled to the bottom of the flask, the upper 
part of the clot being composed of a firm yellowish buffy coat. The 
serum separated well and was very clear. 
The blood serum was drawn off and diluted with an equal volume 
of an isotonic salt solution and then divided into two portions. 
Case. 
Filomena Martinez. 
Estivo-autumnal Malaria. 
Month. 
0 C T O B E R . 
Day of 
Month. 
27 
28 
29 
30 
Day of 
Disease. 
A.M. 
% 
<?) 
C\J 
Co 
CVJ 
Co 
§ 
Co 
s 
P.M. 
CM 
5 
oi 
CVJ 
§ 
CV 
Co 
a 
41 
Co 
0 
3 
>• 
$ 
£ 
ik 
- s 
Jr 
40 
£ 
Oi 
fV 
r. . 
r- 
£ 
- 
-k 
<0 
39 
3 
s 
'cs 
- 
38 
r» 
p 
F 
p 
f: 
5 
v 
CO 
CO 
CO 
C3 
pQ 
K 
\ 
\ 
37 
/ 
> 
> 
/ 
c 
L 
36 
v 
A 
v 
- 
CO 
§ 
Co 
o> 
Co 
Oi 
CM 
Oo 
co 
00 
cvj 
Oi 
CM 
Oi 
Co 
00 
Oi 
CM 
O 
5 
§ 
CD 
03 
k 
00 
°o 
Temperature chart of Filomena Martinez. 
One portion was passed through a Chamberland B filter and in¬ 
jected into Jose Ojeira. 
The other portion was passed through a Berkefeld filter and 
injected into Luis Peredo. 
Blood smears made from the blood which was drawn from the vein 
showed in stained specimens crescents and the young small ring forms 
of estivo-autumnal malaria. Some of the stained parasites showed 
one chromatin point, others two. A few were irregular in outline, 
