84 
This filtration was also done from without inwards, as may be seen 
by reference to the sketch (fig. 3), but by using vacuum instead of 
direct air pressure, as in the case of the filtration through the Pasteur- 
Chamberland bougie. The filtrate was carried over to a sterile bottle, 
as shown in the sketch, and drawn out with a pipette,.so that, care 
being taken, there could be no chance of contamination. It required 
about twenty minutes to filter 46.5 cc. of diluted serum, and at 
8.15 p. m., viz, eight and one-fourth hours after the blood was drawn, 
twenty cubic centimeters of this filtrate, which represented 10 cc. of 
the original serum, were injected into the left median basilic vein of 
Luis Peredo with entirely negative results. 
This filter was then thoroughly washed with water which ran 
through in drops under atmospheric pressure, and was preserved 
for further testing, with the following results: 
On March 1, 1904, this filter was tested with a bouillon culture of Staphylo¬ 
coccus pyogenes aureus . The filtrate remained sterile after ten days in the 
incubator. 
At 10 p. m. Martinez was given 1 gram of bimuriate of quinine 
directly into one of the veins of his arm. This was repeated at noon 
on October 28, and again at 11 p. m. on the same day. 
On the 29th he received another gram into the vein, with marked 
improvement in his symptoms and a notable reduction in the number 
of intracorpuscular forms in the blood, as will be seen by the following 
notes of the case: 
October 27,10 p. m. —One gram bimuriate of quinine intravenously. 
Blood examination, 3 crescents in one field; some fields have as many 
as half a dozen small ring forms. 
October 28 , 2 a. m. —Blood examination. Some fields have 5 and 6 
small ring forms, some irregular amoeboid shapes, also ovoids. 
6 a. m.— Blood examination. One or two ring forms to each field; 
also crescents. 
10 a . m.—Blood examination. About 1 organism to each field; 
some young ring forms; some amoeboid shapes; also ovoids. 
12 noon.— One gram bimuriate of quinine intravenously. 
2 p. m. Blood examination. About 1 intracorpuscular form to 
each field. 
11 p. m. —One gram bimuriate of quinine intravenously. 
October 29 , 2 a. on. —Ten-minute search of a slide stained with 
polychrome methylene blue showed no intracorpuscular forms; cres¬ 
cents not diminished in numbers. 
6 a. m. Only 2 intracorpuscular rings seen; crescents in moderate 
numbers. 
10 a. m .—Given 1 gram bimuriate of quinine into a vein. Five- 
minute search of a stained blood smear shows 5 ovoids and only 1 
intracorpuscular ring form. 
