86 
Present illness dates from about November 3, but states that he 
has been troubled with mild attacks of fever for a month, which he 
describes as coming on alternate days, but not sufficiently severe to 
keep him from his work. 
The fever which initiated his present sickness began with a severe 
chill, and was followed by fever and sweat, and was associated with 
some nausea and vomiting. He states that these paroxysms were 
repeated daily until his admission to San Sebastian Hospital, Novem¬ 
ber 6, 1903. 
Blood examination showed that he had a heavy infection with 
tertian parasites, and he was immediately transferred to the labora¬ 
tory of Working Party No. 2, Yellow Fever Institute. 
The man was physically robust, but very anemic, mucous mem¬ 
branes particularly pale, skin cold and damp. 
At about noon on this date (November 6), the patient was seized 
with a chill. 
By 12.30, half an hour later, the rigor was very marked; he lay in 
bed with a blanket drawn over his head, and was shaking violently; 
he could not hold a thermometer in his mouth, and the pulse was 
taken with difficulty. During this time the temperature was rapidly 
rising, it being now 39.1° C. 
At 12.40 blood was drawn from one of the superficial veins at the 
bend of the elbow. On account of the rigor there was some difficulty 
in introducing the needle. The blood flowed freely; 125 cc. were 
quickly drawn. It was permitted to flow into a porcelain dish and 
immediately defibrinated by whipping with sterilized forks. Clotting 
took place very quickly, so that the fibrin was readily and quickly 
separated from the fluid. 
Judging from the size of the clot and color the fibrin had enmeshed 
a number of corpuscles. The defibrinated fluid showed no further 
tendency to clot, and on microscopical examination looked like fresh 
blood containing a normal number of corpuscles. 
To 25 cc. of defibrinated blood was added 25 cc. of physiological 
salt solution, and this diluted blood was filtered through the same 
Berkefeld filter in the same manner as was done with the blood of 
Filomena Martinez (see p. 84). This filter, when tested later, March 
1, 1904, held back Staphylococcus pyogenes aureus. 
Nine cc. of the filtrate were injected into the right basilic vein of 
Luis Peredo as soon as this amount could be obtained. This injection 
took place at 1.40 p. m. It only took about forty minutes to defibrin- 
ate and filter the blood, which process was done as rapidly as possible. 
Stained smears of the filtrate showed no morphologic elements. 
The filtrate had a distinct red color. For the method by which this 
filtration was done, see fig. 3. 
As a control, Jose Ojeira, at 2 p. m., was given an injection into 
