88 
his left basilic vein of 4 cc. 'of the unfiltered mixture. As the blood 
was diluted with equal parts of salt solution, he therefore received 2 
cc. of Mendez’s blood. 
The unfiltered mixture of defibrinated blood and salt solution, upon 
microscopic examination shortly after Ojeira received his injection, 
showed amoeboid tertain organisms with dancing pigment. For the 
character of the malarial parasites infecting the blood of Mendez, see 
illustration, plate 3. 
After drawing the blood from Mender he continued to have a 
chill, with severe rigor and chattering of the teeth, accompanied by 
nausea and vomiting. His temperature continued to rise after the 
blood was drawn until it reached 40.2° C. The febrile period was 
followed by drowsiness and moisture of the skin. 
As will be seen by reference to the temperature chart, Mendez was 
kept under observation without quinine, and had another typical 
malarial paroxysm the next day. All the evidence in his peripheral 
blood, which was examined frequently, pointed to a severe double 
infection with the tertian parasite. 
He was then given quinine, which entirely controlled the disease, 
and caused the complete disappearance of the parasites from his 
peripheral blood. 
The results caused by the injection of the blood of Andres* Mendez 
into Peredo and Ojeira follow: 
Luis Peredo (case LXIV), a volunteer, aged 25, born in Jalapa, 
State of Vera Cruz, wdiere he has always lived. When examined at 
Jalapa, August 26, he was found to be physically sound; urine con¬ 
tained no albumin; peripheral blood showed no plasmodium. 
He was brought to Vera Cruz August 28 and taken from the station 
directly to the laboratory, from which time he was kept constantly 
within a mosquito-proof room. 
On October 27, after having been under daily observation two 
months, during which time he remained in normal health, he was 
injected with the filtered blood of Filomena Martinez (page 84), who 
at the time was suffering with a paroxysm of malarial fever of the 
estivo-autumnal type, his blood containing many young ring-forms 
and crescents. 
It will be noted by reference to the records of Filomena Martinez 
that the blood was drawn during the decline of the paroxysm. It was 
then allowed to clot in the ice chest; the clear serum was pipetted off 
and diluted with an equal quantity of isotonic salt solution, and this 
filtered through a new Berkefeld filter. 
Twenty cubic centimeters of the filtrate, which on account of the 
dilution represented 10 cc, of the blood serum, were injected into the 
left median basilic vein of Peredo. 
