91 
On August 28, at 9.30 a. m., he was bitten by four mosquitoes, two of 
which had bitten Antonio Leal (case XXXV), a yellow-fever patient 
fifteen days seventeen hours previously; and the other two had bitten 
the same case fourteen days twenty-three hours previously. 
The man was kept under close observation in a mosquito-proof 
room, but showed no reaction. There was no rise of temperature, nor 
did he present any untoward symptoms. 
On October 27, 7.20 p. m., he received intravenously 20 cc. of diluted 
blood serum of Filomena Martinez (sestivo-autumnal infection), 
passed through a Pasteur-Chamberland filter B. This represented 
10 cc. of blood serum. For cletals of this filtration see Filomena 
Martinez (p. 81). 
Ojeira showed no reaction whatever as a result of this injection. 
It will be noted that the blood of Martinez was drawn after the 
height of the paroxysm, and while the temperature was on the decline. 
Martinez was suffering with a very severe sestivo-autumnal infection 
at the time the blood was taken. 
Ojeira's blood was carefully examined several times daily, both be¬ 
fore and following this experiment, and at no time was anything 
resembling a malarial parasite seen in his peripheral blood. 
On Xovember 6, the patient having continued in good health since 
the last experiment, was used as a control for the experiment made on 
Peredo. 
On this date, at 2 p. m., he was given an intravenous injection of 
4 cc. of the unfiltered , diluted, and defibrinated blood of Andres 
Mendez. At the time the blood was drawn from Mendez it contained 
a heavy infection of a double tertian malaria, and the blood was taken 
from him during a chill and before the height of his paroxysm. It 
was at once defibrinated, diluted with an equal volume of physiolog¬ 
ical salt solution, and filtered through a Berkefeld filter. Xine cc. of 
the filtrate were given intravenously to Peredo, causing a malarial 
paroxysm without, however, the presence of. the malarial parasite, 
and due, as we believe, to the toxin (?) in the blood of Mendez. 
Ojeira, who received 2 cc. of unfiltered blood (4 cc. dilution), reacted 
within an hour, with a slight rise of temperature and nausea, and 
four days following developed a typical malarial paroxysm, with 
many tertian parasites in his peripheral blood. 
There can be no doubt that the reaction to the 2 cc. of defibrinated 
blood injected into the vein of Ojeira caused a slight paroxysm, 
which it is reasonable to suppose was due to the same poison present in 
the blood of Mendez, and which also caused the reaction in Peredo. 
It will be noticed that 2 cc. of this blood caused but a slight reac¬ 
tion in the case of Ojeira, while 4.5 cc. caused a more marked reac¬ 
tion, with a rise of temperature to 38.7° C., in the case of Peredo, 
