24 
lene violet. Giemsa’s mixture (procured ready made) 
is then— 
Azur II-Eosin 
Azur II 
3 parts 
o*8 part 
Glycerin 
Methyl alcohol 
250-0 parts 
250*0 „ 
To Stain. —(1) Fix in alcohol. (2) Mix one drop 
of the solution with 1 c.c. of water. (3) Stain five to 
ten minutes, or several hours. (4) Wash in water. 
Note. —To obtain malignant stippling (p. 33) it is 
recommended to add to 10 c.c. of solution one or two 
drops of 1 per mille potassium carbonate solution. 
To obtain the most brilliant results with these 
stains is perfectly easy, and no one who has used them 
will, except for special reasons, use any others at 
present in use. 
As stated above the simple Romanowsky stain is 
as good as any of the modifications. 
To make fresh films. —For studying movement, 
delicacies of structure, for watching the process of 
ex-flagellation, phagocytosis, fertilisation, and other 
phenomena of the living parasite, it is necessary to be 
able to make good fresh films. 
It is well to polish the slide and coverglass immedi¬ 
ately before use with a clean handkerchief. When the 
exuding drop of blood reaches the size of a small pin’s 
head, a coverglass is picked up rapidly with forceps, or 
the edges are grasped between finger and thumb, and 
allowed to touch the drop without 4 dabbing ’ the skin, 
and then carefully dropped on to a slide. A gentle tap 
or two with a needle or forceps may aid in the film 
formation, but the pressure must not be great or the 
corpuscles will be found laked and invisible. 
The requirements of a suitable wet film are. —That 
