4i 
concentrated into a ring or figure of 8. Observe that 
attached to the:e spherical bodies small ring-like 
bodies occur, one to two in number, about as big as a 
pin’s head. These are the so-called 4 polar ’ bodies. 
They occur in the male and female, and, as seen in 
stained specimens, consist of little circular masses of 
chromatin (?). These changes occur in the tropics 
very rapidly, so that the examination must be com¬ 
menced as rapidly as possible. 
On watching these spheres, the pigment in some 
will be seen to be in active motion—this probably 
indicates the internal changes preparatory to extrusion 
of flagella. Suddenly one of these spheres is per¬ 
ceived to be oscillating violently, and in a moment 
three or four or more pale, long processes are emitted 
(Fig. 9). The red cells all around are put in motion 
by their violence, and it may be only after a time, 
when the activity has grown less, that the flagella are 
actually seen. Nodosities will be observed in the 
flagella, and occasionally a speck of pigment at their 
extreme end. The flagella after a time break off, but 
they have only once, by MacCallum, been seen 
penetrating the female gamete. 
To Stain Flagellated Bodies 
When flagellation is observed the coverglass is 
forcibly 4 smeared ’ off ; slide and coverglass are then 
fixed, and stained with Romanowsky. 
Beautiful preparations are easily got by this 
method (Fig. 9). 
The Subsidiary Signs of Malaria 
When patients have taken quinine it is not 
uncommonly impossible to find parasites in the peri- 
