44 
Chaffer IV 
The Parasite in the Tissues 
Fo Make Smear Preparations. —Place a minute 
portion of the tissue {e.g. brain, spleen) on a slide, and 
with the end of another slide spread it out as evenly 
and thinly as possible. Dry, fix, and stain in the same 
way as a blood film. Parasites, if present, are in this 
way much more easily and clearly seen than in sections. 
Spleen pulp, bone marrow, kidney, liver, etc., give 
beautiful results, and in the same way any secretion 
or fluid can be examined. 
Fixation of Fissues. —N.B.—Use small pieces. 
1. Cut the tissues with a sharp knife or razor into 
pieces not thicker than one-eighth of an inch (3 mm.) 
2. Place some cotton wool at the bottom of a 
bottle or specimen tube. Pour in the fixative and 
add the pieces of tissue. The amount of fixative to 
be used should be about fifty times the volume of 
the tissue. 
3. Pieces of intestine (< e.g ., in cases of malaria 
cholerica ) may be laid on pieces of paper and then put 
in the fixative. The tissue adheres to the paper and 
retains its shape. Proceed similarly for any thin tissue 
or thin slice of an organ which it is required to embed 
and get sections of rapidly. 
4. The results got with alcohol or formalin are 
not nearly as good as with other fixatives which require 
some trouble in the making. ( Vide ch. xxxiv.) 
