4 8 
the slide is covered with a series of ribbons (if desired) 
one beneath the other. Warm the sections very care¬ 
fully over a small flame until the paraffin is stretched. 
4. Drain off the excess of water and then blot 
off the remainder. Dry the sections in an ordinary 
incubator 38'5° (about;; or by a fire, protected from 
dust; or, for rough purposes, over a flame without 
melting the paraffin wax. 
5. Allow xylol to trickle over the section till the 
paraffin is dissolved out, or place in a pot of xylol. 
6. Wash off the xylol in alcohol and then bring 
(through descending strengths of alcohol) into water. 
{Vide ch. xxxiv.) 
Staining 
Haematein .—Stain five to twenty minutes accord¬ 
ing to the activity of the stain as seen by the depth of 
colour of the sections. If over-stained they may be 
decolorized in one per cent, alum solution. Wash in 
water. Counterstain with eosin (p. 408) for one 
minute. 
Methylene Blue .—Stain with one-fourth per cent, 
methylene blue for one hour. Wash in water and 
alcohol and counterstain with eosin. 
Romanozvsky .—It is difficult to get good results 
with sections as indeed it is with thick blood films. 
Formalin should not be used to fix. The sections 
should be as thin as possible. Fulleborn recommends 
the following method. 
(1) Giemsa stain, 10 drops, H 2 0 20 c.c. Stain 
with two or three lots for several hours. 
(2) Wash off excess and then drop on acetic acid, 
1 in 1000, till violet. Wash in water. 
