49 
(3) Stain with the ordinary diluted Romanowsky 
(for one hour). 
(4) Place in seventy per cent, alcohol and gradually 
increase strength till absolute alcohol containing some 
eosin is added. 
(5) Xylol. Balsam. 
To Mount Sections .—Pass through successive 
alcohols, oil of cloves (or xylol), to Canada balsam. 
In hot moist climates, the cold produced by the 
evaporation of the alcohol causes dew to be deposited 
upon the slide. When the xylol or oil of cloves is 
added, this produces a troublesome milkiness and may 
spoil the section. To avoid this, all excess should be 
rapidly wiped up after the use of alcohol, and the oil 
of cloves added as quickly as possible. 
Post-Mortem Changes in Malaria 
(Marchiafava and Bignami) 
Brain :— 
1. Punctiform haemorrhages of the meninges. 
2. Punctiform haemorrhages of the white sub¬ 
stance of the brain. 
3. The brain capillaries may have nearly every 
red cell infected. Sporulating forms are especially 
common. 
4. The capillary endothelium may show fatty 
degeneration, together with pigmentation, and some¬ 
times parasites. 
5. Similar appearances are also found in the 
vessel of the pia mater. 
Lungs :— 
1. Large pigmented mononuclears in the 
capillaries, but especially in the veins ; in the lungs 
especially, phagocytosis is proceeding. 
E 
