some Anophelines with, ripe ovaries are placed. Observe 
that ova are laid upon the mud. 
7. Preserve the mud for forty-eight hours, pre¬ 
venting it from becoming completely dry. 
At the end of forty-eight hours or more, remove 
a few ova to a dry slide, and place under a low power. 
Allow a drop of water to flow on to the ova. Observe 
the escape, within a minute or so, of the young larvae, 
and the fact that a cap-like piece of the egg-shell is 
pushed off. 
8. Observe that Anophelines kept in a dry test 
tube will occasionally lay their eggs on the side of the 
tube. 
9. Note the time when the eggs were laid and 
the time at which the larvae emerge. This depends 
greatly on the temperature. It may take two or three 
days. Ce. argyrotarsis , one-and-a-half days (Taylor). 
10. Remove Anopheline ova on paper and allow 
them to dry, and note that after two, or three days 
(86°"96° F.) at the most, they will not hatch when 
carefully placed in water. 
