92 
board as above, over a bottle in which Anofhelines , 
caught in a village or elsewhere, have been placed. 
Remove the cotton plug and shake the bottle gently 
to drive the insects out. Replace jar upon the pre¬ 
pared stopper. Place in a dark spot. Next morning 
remove the stopper and remove any dead mosquitoes 
and ova by taking out the piece of paper. 
On the second night after the mosquitoes have 
been collected, the bottle is ready for feeding experi¬ 
ments. On the third day, generally, the mosquitoes 
have no longer any blood remaining in the mid-gut, 
and are ready for dissection. 
The glands of any mosquitoes that may die before 
this may of course be dissected, if desired, on the chance 
of finding sporozoits. 
In the use of village-caught Anophelines , it must 
be borne in mind that any subject upon which they 
are fed is liable to a fresh infection. In the case of 
natives (who sleep without hesitation in any village), 
the employment of village-caught mosquitoes cannot, 
however, be very prejudicial. 
The advantages of the above way of keeping 
mosquitoes are :— 
1. The mosquitoes will keep alive longer than 
in any other way known to us. 
2. The immense convenience in feeding. 
3. Any mosquitoes that may have died in the 
night can be recovered, and are not dried up. 
4. It is an extremely convenient way of obtaining 
and examining the ova. 
5. Mosquitoes which have become feeble are 
given the best possible chance of living, and will be 
found resting all day on the piece of paper. 
If boxes and net-covered frames be used, an 
enormous mortality usually results. The dead bodies 
