94 
this around the neck of the bottle with twine. The 
bottle is then ready for feeding. 
At, or shortly after dark, take as many bottles as 
may be desired to the ward or dormitory. Slightly 
damp the forearm (or the calf) of the patient, and, 
turning the bottle right side up, let the patient’s 
arm rest upon the mouth of the bottle (Fig. 36). 
In from a few minutes to half-an-hour or more 
the bottle will be noticed to have splashes of blood 
upon the bottom and sides (in the case of Anophelines 
only). If possible wait till all the Anophelines have fed.- 
Remove the bottle, invert upon a table, untie the 
twine, and remove the netting. Replace the bottle 
upon the prepared stopper. 
Repeat the process every night, allowing the 
mosquitoes to feed by preference on the same case 
throughout. 
Add clean water and a fresh piece of paper each 
time the bottle is used. 
To Prepare Fed Mosquitoes for Dissection 
After having fed the mosquitoes in a bottle for 
a certain number of days, allow them to remain 
undisturbed (merely changing the water, etc.) for 
several days. 
Ascertain each day whether the mosquitoes have 
completely got rid of the blood in the mid-gut. When 
they are quite free from anv dark colouration of the 
ventral aspect of the abdomen they are ready for 
dissection. 
N.B.—If chloroform, and especially if tobacco smoke is used to 
kill the mosquito, it is essential to well wash the jars before again 
keeping mosquitoes in them. 
