IOI 
To Prepare the Mid-Gut for Examination 
1. Cut (by pressing with, the needle) across the 
intestine and malpighian tubes just below the termi¬ 
nation of the saccular mid-gut. This will separate 
the mid-gut from the rest of the viscera. 
2. Remove everything from the slide but the 
mid-gut. Remove excess of fluid, and see that no 
ova or extraneous matters are left upon the slide. 
Add a small drop of clean salt solution, and place a 
thin coverglass upon the preparation. The mid-gut 
will flatten out considerably. Remove with filter 
paper applied to the edge of the coverglass any excess 
of fluid. Examine under one-third inch objective and 
afterwards under one-twelfth. 
If the mid-gut has been removed in toto , and the 
preparation not too much compressed, the following 
appearances are seen :— 
1. The narrow anterior portion of the mid-gut 
with the calyx-like proventriculus at its free end. 
2. If a portion of the extremely thin membrane 
of the true oesophagus or its diverticula be included 
in the preparation, it will probably be seen to exhibit 
peculiar markings, due probably to muscular fibres 
in the membrane, but resembling rather closely 
sporozoits. It is essential that this structure should 
be recognised when seen, and that the resemblance 
of its markings to sporozoits should not lead the 
beginner astray (Fig. 40). 
3. The expanded posterior portion of the mid- 
gut. This body forms the main mass of the prepara¬ 
tion, and is all important in relation to malarial studies. 
The following appearances are seen in a good 
preparation :— 
1. Well-defined tubes with spiral lining (air 
