104 
remains in the mid-gut (for methods of keeping alive 
see p. 90). 
Prepare the mid-gut as described above. A con¬ 
siderable number map prove negative, but a variable 
percentage will be positive. Examine with one- 
twelfth inch. 
Carefully note the presence of small collections 
of pigment of the nature of malarial figment. Bp 
careful focussing, the younger forms map be seen as 
clear oval or round bodies, 6-7/4, in which the distinct 
clearly defined pigment occurs. The more advanced 
forms can scarcely be missed. It is necessary to bear 
in mind the normal structures and the fact that, until 
the parasite reaches a considerable size and has a very 
sharply defined cyst wall, pigment, of the character 
belonging to the species of parasite concerned, is 
present. 
Fig. 40. {Left to right) Pericardial Cell, Fat-Body Cell, Swollen 
Epithelium Cell , Diverticulum shewing Sporozoit-like appearance 
1. Zygotes of malignant tertian shew, when 
young, a clump of pigment resembling black pepper 
grains (Fig. 41). 
2. Zygotes of simple tertian shew yellowish 
or golden pigment in wisps (Fig. 41). 
3. Zygotes of quartan shew rather coarse pigment 
in a clump (Fig. 41). 
The older zygotes (40-60/4) are indistinguishable 
