io5 
as regards the species of parasite concerned. They 
map shew :— 
1. A very clear and distinct oocyst wall (adven¬ 
titious). 
2. The formation of sporoblasts. 
3. In still more developed forms, the sporoblasts 
are seen to be surrounded by a radiating arrangement 
of young sporozoits or blasts (Figs. 14 and 39). 
4. Fully developed forms are large cysts packed 
with many hundreds of fine sickle-shaped bodies, and 
if they are ruptured, these latter escape into the 
surrounding fluid, and are readily distinguished with 
a sixth-inch lens as sporozoits (Fig. 45). 
Fig. 41. {Left to right ) Zygotes of Malignant Fertian , Simple 
Tertian , Quartan , and Proteosoma 
To Make Permanent Preparations of 
Zygotes 
Method 1.—In case of a specimen shewing zygotes, 
place a large drop of ten per cent, formalin on one 
side of the coverglass, and draw this through by filter 
paper placed on the other side. Repeat several times. 
Remove excess of the formalin with filter paper. Seal 
edges of coverglass first with glycerine jelly and then 
with cement (p. 408). 
The zygotes will retain their appearance as seen 
in the fresh specimen. 
Method 2.—Run formalin through as in Method 1. 
