log 
This method, which is longer than Method I, 
requires more dissecting and teasing out in order to 
isolate the glands cleanly, and, as we have said, may 
still be followed, even if No. I has failed ; but our 
experience has been that Method i is learned at once 
without any difficulty. 
Fig. 44. Microscopic Structure of Salivary Acinus and that of a Newly- 
Hatched Mosquito (right) 
Ascertain that the glands of either side consist 
of three acini, the ducts of which join almost imme¬ 
diately after leaving the acinus to form a single long 
duct. 
1. Observe that of the three glands of each side 
(Figs.. 43 and 46) 
(i) Two are highly refractive, and the cells in 
these are very distinct and clearly defined (lateral 
glands). 
(ii) One is much less refractive, and the com¬ 
ponent cells are much less easily defined (central gland). 
2. Observe that each acinus has a duct running 
through its whole length, and that the secretory cells 
form a single row around this. 
3. Observe that each secretory cell has a large 
mass of clear secretion within it, forming the chief 
bulk of the cell, and that the nucleus is flattened and 
pushed to the periphery (Fig. 44). Pressure tends to 
